Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways
| Autor: | Enrique Querol, Lucía Sánchez-Alba, Alex Perálvarez-Marín, Sergi Torres-Puig, Carlos Martínez-Torró, Jaume Piñol, Miguel González-Martín, Francesc Canals, Oscar Q. Pich, Marina Marcos-Silva, Marta Monge |
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| Přispěvatelé: | Institut Català de la Salut, [Martínez-Torró C, Torres-Puig S, Sánchez-Alba L, González-Martín M, Marcos-Silva M] Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Monge M, Canals F] Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Models Molecular Proteomics Metal acquisition systems Operator (biology) Transcription Genetic Epidemiology Operon Mutant Mycoplasma genitalium Conserved sequence Histidine-rich proteins 2 2'-Dipyridyl Microorganismes patògens Drug Discovery Homeostasis Promoter Regions Genetic Sexually transmitted diseases integumentary system General Medicine Cell biology Emerging STI pathogen Infectious Diseases Malalties bacterianes gramnegatives Pathogenic microorganisms Novel therapeutic targets metal acquisition systems Bacteria::bacterias gramnegativas::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitalium [ORGANISMOS] fenómenos genéticos::regulación de la expresión génica::regulación de la expresión génica bacteriana [FENÓMENOS Y PROCESOS] Malalties de transmissió sexual Ferric uptake regulator inorganic chemicals Bacteria::Gram-Negative Bacteria::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitalium [ORGANISMS] Metallome Iron 030106 microbiology Immunology Mutagenesis (molecular biology technique) Biology Microbiology Article 03 medical and health sciences metallome Bacterial Proteins Virology Regulació genètica Genetic Phenomena::Gene Expression Regulation::Gene Expression Regulation Bacterial [PHENOMENA AND PROCESSES] Micoplasmes Mycoplasmatales Amino Acid Sequence Gene novel therapeutic targets Reporter gene Promoter Gene Expression Regulation Bacterial emerging STI pathogen Repressor Proteins 030104 developmental biology bacteria Parasitology |
| Zdroj: | Emerging Microbes & Infections Dipòsit Digital de la UB Universidad de Barcelona Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Scientia |
| ISSN: | 2222-1751 |
| Popis: | Regulador d'absorció fèrric; Mycoplasma genitalium; Patogen emergent d'ITS Regulador de la absorción férrico; Mycoplasma genitalium; Patógeno emergente de ITS Ferric uptake regulator; Mycoplasma genitalium; Emerging STI pathogen Transition metals participate in numerous enzymatic reactions and they are essential for survival in all living organisms. For this reason, bacterial pathogens have evolved dedicated machineries to effectively compete with their hosts and scavenge metals at the site of infection. In this study, we investigated the mechanisms controlling metal acquisition in the emerging human pathogen Mycoplasma genitalium. We observed a robust transcriptional response to metal starvation, and many genes coding for predicted lipoproteins and ABC-transporters were significantly up-regulated. Transcriptional analysis of a mutant strain lacking a metalloregulator of the Fur family revealed the activation of a full operon encoding a putative metal transporter system and a gene coding for a Histidine-rich lipoprotein (Hrl). We recognized a conserved sequence with dyad symmetry within the promoter region of the Fur-regulated genes. Mutagenesis of the predicted Fur operator within the hrl promoter abrogated Fur- and metal-dependent expression of a reporter gene. Metal starvation still impelled a strong transcriptional response in the fur mutant, demonstrating the existence of Fur-independent regulatory pathways controlling metal homeostasis. Finally, analysis of metal accumulation in the wild-type strain and the fur mutant by ICP-MS revealed an important role of Fur in nickel acquisition. This work was supported by the Ministerio de Ciencia, Innovación y Universidades (Grant BIO2017-84166-R). The Proteomics Unit at VHIO is a member ProteoRed, PRB3 (Grant IPT17/0019 – ISCIII-SGEFI/ERDF). |
| Databáze: | OpenAIRE |
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