Pullulan dialdehyde crosslinked gelatin hydrogels with high strength for biomedical applications

Autor: Liru Zhang, Xianliang Zhang, Xuejing Zheng, Jie Liu, Ali Zhang, Keyong Tang
Rok vydání: 2019
Předmět:
congenital
hereditary
and neonatal diseases and abnormalities
food.ingredient
Compressive Strength
Polymers and Plastics
education
Biocompatible Materials
macromolecular substances
02 engineering and technology
010402 general chemistry
01 natural sciences
Gelatin
Cell Line
Mice
chemistry.chemical_compound
food
Elastic Modulus
health services administration
Materials Chemistry
medicine
Animals
Collagenases
Fourier transform infrared spectroscopy
Glucans
Aldehydes
Hydrolysis
Periodic Acid
Organic Chemistry
technology
industry
and agriculture
Hydrogels
Pullulan
021001 nanoscience & nanotechnology
0104 chemical sciences
Cross-Linking Reagents
Compressive strength
chemistry
Chemical engineering
Covalent bond
Ninhydrin
Self-healing hydrogels
Swelling
medicine.symptom
0210 nano-technology
Oxidation-Reduction
Porosity
Zdroj: Carbohydrate Polymers. 216:45-53
ISSN: 0144-8617
Popis: Herein the construction of a strong gelatin hydrogel is presented by using pullulan dialdehyde (PDA) as a macromolecular crosslinker. The resultant PDA crosslinked gelatin hydrogels (G-PDA) exhibit extremely high mechanical strength, manifested in the achieved optimal compressive stress of 5.80 MPa at 80% strain, which is up to 152 times higher than pure gelatin hydrogel. The G-PDA were characterized by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The extent of crosslinking was determined by ninhydrin assay. The results suggested that the synergistic effect of dual-crosslinking, which is composed of short- and long-range covalent crosslinking and thermoreversible physical crosslinking, may played a key role in enhancing the load-bearing capacity of ensuing hydrogels. The swelling and enzymatic degradation of G-PDA are gradually limited with increasing PDA concentration. The result from MTT assay demonstrated that G-PDA is non-cytotoxic against MC3T3 cells, regardless of the concentrations of PDA.
Databáze: OpenAIRE
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