Pullulan dialdehyde crosslinked gelatin hydrogels with high strength for biomedical applications
Autor: | Liru Zhang, Xianliang Zhang, Xuejing Zheng, Jie Liu, Ali Zhang, Keyong Tang |
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Rok vydání: | 2019 |
Předmět: |
congenital
hereditary and neonatal diseases and abnormalities food.ingredient Compressive Strength Polymers and Plastics education Biocompatible Materials macromolecular substances 02 engineering and technology 010402 general chemistry 01 natural sciences Gelatin Cell Line Mice chemistry.chemical_compound food Elastic Modulus health services administration Materials Chemistry medicine Animals Collagenases Fourier transform infrared spectroscopy Glucans Aldehydes Hydrolysis Periodic Acid Organic Chemistry technology industry and agriculture Hydrogels Pullulan 021001 nanoscience & nanotechnology 0104 chemical sciences Cross-Linking Reagents Compressive strength chemistry Chemical engineering Covalent bond Ninhydrin Self-healing hydrogels Swelling medicine.symptom 0210 nano-technology Oxidation-Reduction Porosity |
Zdroj: | Carbohydrate Polymers. 216:45-53 |
ISSN: | 0144-8617 |
Popis: | Herein the construction of a strong gelatin hydrogel is presented by using pullulan dialdehyde (PDA) as a macromolecular crosslinker. The resultant PDA crosslinked gelatin hydrogels (G-PDA) exhibit extremely high mechanical strength, manifested in the achieved optimal compressive stress of 5.80 MPa at 80% strain, which is up to 152 times higher than pure gelatin hydrogel. The G-PDA were characterized by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The extent of crosslinking was determined by ninhydrin assay. The results suggested that the synergistic effect of dual-crosslinking, which is composed of short- and long-range covalent crosslinking and thermoreversible physical crosslinking, may played a key role in enhancing the load-bearing capacity of ensuing hydrogels. The swelling and enzymatic degradation of G-PDA are gradually limited with increasing PDA concentration. The result from MTT assay demonstrated that G-PDA is non-cytotoxic against MC3T3 cells, regardless of the concentrations of PDA. |
Databáze: | OpenAIRE |
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