Inhibition of Tissue-Bound Semicarbazide-Sensitive Amine Oxidase by Two Haloamines, 2-Bromoethylamine and 3-Bromopropylamine
| Autor: | Kaori Takayanagi, Kensuke Kisara, Hiroyasu Kinemuchi, Yuichiro Arai, Naoyuki Kobayashi, Takeshi Tadano, Kazuya Takahashi, Lars Oreland |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Alkylating Agents Amine oxidase Time Factors Monoamine oxidase Kinetics Biophysics Inhibitory postsynaptic potential Biochemistry Adduct Rats Sprague-Dawley Ethylamines Animals Enzyme Inhibitors Lung Molecular Biology chemistry.chemical_classification Dose-Response Relationship Drug Propylamines Amine oxidase (copper-containing) Rats Enzyme chemistry Covalent bond Amine Oxidase (Copper-Containing) |
| Zdroj: | Archives of Biochemistry and Biophysics. 385:154-161 |
| ISSN: | 0003-9861 |
| Popis: | Various mammalian tissues contain membrane-bound amine oxidase termed semicarbazide-sensitive amine oxidase (SSAO). A variety of compounds has been identified as relatively selective SSAO inhibitors, but those inhibitors currently available also inhibit monoamine oxidase (MAO). In the present study, inhibitory properties of 2-bromoethylamine (2-BEA) and 3-bromopropylamine (3-BPA) toward rat lung-bound SSAO have been studied. Regardless of preincubation, 2-BEA could not appreciably inhibit MAO-A and MAO-B activity, but 3-BPA at relatively high concentrations inhibited only MAO-B activity. 3-BPA was a competitive and reversible SSAO inhibitor with a Ki value of 17 microM regardless of preincubation. In contrast, without preincubation, 2-BEA competitively inhibited SSAO activity with the Ki value of 2.5 microM and after preincubation, the mode of inhibition changed to be noncompetitive, indicating irreversible inhibition after the preincubation. Dialysis experiments with 2-BEA-pretreated homogenate resulted in no recovery of SSAO activity even after overnight dialysis. A decreased rate of SSAO inhibition under N2 atmosphere to that obtained under O2 was produced upon preincubation of enzyme with 2-BEA, suggesting that oxidized intermediate was necessary for its inhibitory activity. Thus, 2-BEA first interacts with SSAO to form a reversible complex with a subsequent reaction, leading this complex to the covalently bound enzyme-inhibitor adduct. The data analyzed by the plot of 1/k' vs 1/2-BEA concentrations intersected on the y-axis indicate that the inhibition by 2-BEA is not mediated by a bimolecular reaction; thus it is not an affinity-labeling agent, but a suicide SSAO inhibitor. 2-BEA may be employed as a useful compound in the studying SSAO. |
| Databáze: | OpenAIRE |
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