Convenient and versatile subcellular extraction procedure, that facilitates classical protein expression profiling and functional protein analysis
| Autor: | Afsaneh Abdolzade-Bavil, Lothar Goretzki, Jonas Anders, Michaela Kröger, Robertus Hendriks, Scott Hayes |
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| Rok vydání: | 2004 |
| Předmět: |
Proteome
Cells Blotting Western Pilot Projects Biology Cell Fractionation Sensitivity and Specificity Biochemistry Cytosol Cell Line Tumor Native state Humans Electrophoresis Gel Two-Dimensional Molecular Biology Polyacrylamide gel electrophoresis Cell Nucleus Organelles Isoelectric focusing NF-kappa B Membrane Proteins Proteins Subcellular localization Protein subcellular localization prediction Enzymes Cell biology DNA-Binding Proteins Cytoskeletal Proteins Microscopy Fluorescence Membrane protein Electrophoresis Polyacrylamide Gel Isoelectric Focusing Cell fractionation Subcellular Fractions |
| Zdroj: | PROTEOMICS. 4:1397-1405 |
| ISSN: | 1615-9861 1615-9853 |
| DOI: | 10.1002/pmic.200300710 |
| Popis: | Standardized sample preparation to reduce proteome complexity facilitates subsequent proteome analysis. Here we describe a robust sequential extraction method that enables simple fractionation of proteins in their native state according to their subcellular localization, yielding four subproteomes enriched in (a) cytosolic; (b) membrane and membrane organelle-localized; (c) soluble and DNA-associated nuclear and (d) cytoskeletal proteins. Efficiency and selectivity is demonstrated by morphological-, two-dimensional electrophoresis image-, immunological- as well as enzymatic-analysis. In pilot studies, subcellular redistribution of regulatory proteins was successfully measured. |
| Databáze: | OpenAIRE |
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