Transforming growth factor-β (TGF-β) maintains follicular ultrastructure and stimulates preantral follicle growth in caprine ovarian tissue cultured in vitro
| Autor: | V.R.P. Barros, C.A.P. Lopes, Cláudio Cabral Campello, S. V. Castro, S.L. Costa, G.Q. Rodrigues, I.M.T. Lima, José Ricardo de Figueiredo, Sônia Nair Báo, R. Rossetto, Maria Helena Tavares de Matos, Roberta Nogueira Chaves |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
desenvolvimento in vitro
TGF-β medicine.medical_specialty General Veterinary Ovarian Cortex cabra goat Alpha (ethology) Biology In vitro Tissue culture Endocrinology Cell culture Internal medicine Follicular phase folículo pré-antral Ultrastructure medicine preantral follicle in vitro development lcsh:Animal culture Transforming growth factor lcsh:SF1-1100 |
| Zdroj: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 66, Iss 2, Pp 411-416 (2014) Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.66 n.2 2014 Arquivo brasileiro de medicina veterinária e zootecnia Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
| ISSN: | 1678-4162 |
| Popis: | The objectives of this study were to investigate whether TGF-β affect the survival, activation and further growth of goat primordial follicles enclosed in ovarian cortex after in vitro culture. Goat ovaries were collected from an abattoir and pieces of ovarian tissues were cultured for one or seven days in a supplemented alpha Minimum Essential Medium, alone or containing TGF-β (1, 5, 10 or 50ng/mL). Ovarian tissues from the fresh control as well as those cultured were processed for histological and ultrastructural studies. The results showed that when compared with fresh control, there was decrease in the percentages of histologically normal follicles in all treatments only after seven days culture. TGF-β did not affect the activation of preantral follicles regardless of its concentration, however, larger follicles diameter (P |
| Databáze: | OpenAIRE |
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