Functional Characterization of AbeD, an RND-Type Membrane Transporter in Antimicrobial Resistance in Acinetobacter baumannii
| Autor: | Govindan Rajamohan, Manjunath Venkataramaiah, Amitabha Mondal, Vijaya Bharathi Srinivasan |
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| Rok vydání: | 2014 |
| Předmět: |
Acinetobacter baumannii
medicine.drug_class Antibiotics Molecular Sequence Data lcsh:Medicine Gene Expression Drug resistance Microbial Sensitivity Tests Microbiology Fight-or-flight response Antibiotic resistance Bacterial Proteins Intensive care Drug Resistance Bacterial medicine Escherichia coli Amino Acid Sequence lcsh:Science Promoter Regions Genetic Multidisciplinary biology Membrane transport protein lcsh:R Membrane Transport Proteins Transporter Gene Expression Regulation Bacterial Hydrogen Peroxide biology.organism_classification Anti-Bacterial Agents Oxidative Stress biology.protein lcsh:Q Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 10, p e0141314 (2015) |
| ISSN: | 1932-6203 |
| Popis: | Background Acinetobacter baumannii is becoming an increasing menace in health care settings especially in the intensive care units due to its ability to withstand adverse environmental conditions and exhibit innate resistance to different classes of antibiotics. Here we describe the biological contributions of abeD, a novel membrane transporter in bacterial stress response and antimicrobial resistance in A. baumannii. Results The abeD mutant displayed ~ 3.37 fold decreased survival and >5-fold reduced growth in hostile osmotic (0.25 M; NaCl) and oxidative (2.631 μM–6.574 μM; H2O2) stress conditions respectively. The abeD inactivated cells displayed increased susceptibility to ceftriaxone, gentamicin, rifampicin and tobramycin (~ 4.0 fold). The mutant displayed increased sensitivity to the hospital-based disinfectant benzalkonium chloride (~3.18-fold). In Caenorhabditis elegans model, the abeD mutant exhibited (P |
| Databáze: | OpenAIRE |
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