Genotypic and environmental effects on the level of ascorbic acid, phenolic compounds and related gene expression during pineapple fruit development and ripening
| Autor: | Marie Darnaudery, Jacques Joas, Patrick Fournier, Mathieu Lechaudel, Thierry Joët |
|---|---|
| Přispěvatelé: | Démarche intégrée pour l'obtention d'aliments de qualité (UMR Qualisud), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de La Réunion (UR)-Université de Montpellier (UM)-Avignon Université (AU)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Fonctionnement agroécologique et performances des systèmes de cultures horticoles (UPR HORTSYS), Diversité, adaptation, développement des plantes (UMR DIADE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]) |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Non-climacteric ripening Antioxidant Genotype Physiology F60 - Physiologie et biochimie végétale medicine.medical_treatment Plant Science Oxidative phosphorylation [SDV.BID]Life Sciences [q-bio]/Biodiversity Ascorbic Acid Ananas Environment Real-Time Polymerase Chain Reaction 01 natural sciences Polyphenol oxidase F30 - Génétique et amélioration des plantes 03 medical and health sciences Phenols Gene Expression Regulation Plant Gene expression Genetics medicine Q04 - Composition des produits alimentaires 2. Zero hunger Phenylpropanoid biology Reverse Transcriptase Polymerase Chain Reaction Pineapple food and beverages Gene Expression Regulation Developmental Ripening Ascorbic acid biology.organism_classification [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding 030104 developmental biology Biochemistry Fruit Cultivar Reactive oxygen species Reactive Oxygen Species 010606 plant biology & botany |
| Zdroj: | Plant Physiology and Biochemistry Plant Physiology and Biochemistry, 2018, 130, pp.127-138. ⟨10.1016/j.plaphy.2018.06.041⟩ |
| ISSN: | 1873-2690 0981-9428 |
| DOI: | 10.1016/j.plaphy.2018.06.041⟩ |
| Popis: | International audience; Pineapple (Ananas comosus(L.) Merr.) is a non-climacteric tropical fruit whose ripening could be accompaniedby oxidative processes and the concurrent activation of enzymatic and non-enzymatic reactive oxygen species(ROS) scavenging systems. To better understand the variability of these processes among climatic environmentsor genotypes in pineapple, the temporal expression dynamics for genes encoding oxidative and antioxidativestress enzymes were analyzed by real-time RT-PCR during fruit development and ripening, among three culti-vars: Queen Victoria, Flhoran 41 and MD-2 hybrid, and in two climatic areas. Pineapple development andripening involved changes in the levels of transcripts encoding for polyphenol oxidase and transcripts involvedin thefirst steps of the phenylpropanoid pathway and in the balance of ROS, especially those encoding forascorbate peroxydase and metallothioneins, regardless of the cultivar. Our results confirm the same dynamic ingene expression from the two environmental crop areas, however climatic conditions influenced the level of theexpression of the major transcripts studied that were linked to these oxidative and antioxidant metabolisms.MT3a and MT3b transcripts were not influenced by genetic factor. The genetic effect was not significant on thevarious transcripts linked to thefirst steps of the phenylpropanoid pathway and to phenol oxidation, except 4CLones. In ripe pineapple, highly significant relationships were found between the contents in antioxidant meta-bolites, i.e., ascorbic acid and total phenolic compounds, and the transcript levels of genes involved in theenzymatic ROS-scavenging system and in the biosynthesis or regeneration of ROS-scavenging compounds, likephenylpropanoids, ascorbic acid, metallothioneins |
| Databáze: | OpenAIRE |
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