Disturbed in vitro adrenergic modulation of cytokine production in inflammatory bowel diseases in remission
| Autor: | A. Lucas, Annemieke Kavelaars, Sigrid Elsenbruch, Gustav Dobos, Guido Gerken, Cobi Jacoba Johanna Heijnen, Sebastian Haag, J. Langhorst, Pieter M. Cobelens, Gerald Holtmann, Manfred Schedlowski |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Diarrhea Lipopolysaccharides Agonist medicine.medical_specialty medicine.drug_class medicine.medical_treatment Immunology Terbutaline Adrenergic In Vitro Techniques Infections Dexamethasone Monocytes Internal medicine medicine Humans Immunology and Allergy Glucocorticoids Irritable bowel syndrome Dose-Response Relationship Drug Tumor Necrosis Factor-alpha business.industry Remission Induction Adrenergic beta-Agonists Middle Aged Inflammatory Bowel Diseases medicine.disease Pathophysiology Gastroenteritis Interleukin-10 Cytokine Endocrinology Neurology Neurology (clinical) business Glucocorticoid medicine.drug Psychoneuroimmunology |
| Zdroj: | Journal of Neuroimmunology. 182:195-203 |
| ISSN: | 0165-5728 |
| Popis: | Psychological stress has been implicated in the pathophysiology of both inflammatory and functional gastrointestinal (GI) diseases. The goal of this study was to address neuroendocrine modulation of cytokine production by peripheral blood cells in GI diseases.We analyzed the in vitro effects of the beta-adrenergic agonist terbutaline and the glucocorticoid agonist dexamethasone on TNF-alpha and IL-10 production by LPS-stimulated monocytes in whole cell blood cultures in patients with inflammatory bowel diseases in remission (N=10), diarrhoea-predominant irritable bowel syndrome (IBS, N=12), patients with a recent gastroenteritis (post-infectious group, N=10), and healthy controls (N=15).In response to terbutaline, there was a significant increase in IL-10 production (concentration effect: p0.05), which was diminished in IBD (group effect: p0.01), comparable in IBS and controls, but enhanced in the post-infectious group (group x concentration effect: p0.05). In contrast, terbutaline resulted in a concentration-dependent suppression of TNF-alpha production, which was comparable in all groups. Dexamethasone suppressed TNF-alpha production in a dose-dependent manner in all groups, but this effect was significantly more pronounced in post-infectious subjects (group effect: p0.05).In IBD, disturbed adrenergic regulation of IL-10 could be part of the mechanism(s) underlying the modulation of disease activity by psychological stress. Diarrhoea-predominant IBS was not associated with altered adrenergic or glucocorticoid regulation of cytokine production by peripheral blood cells, whereas a recent history of gastroenteritis was associated with disturbed neuroendocrine modulation of cytokine production, which may play role in the pathophysiology of post-infectious IBS. |
| Databáze: | OpenAIRE |
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