Sox9 function in salivary gland development
| Autor: | Kenji Mishima, Rika Yasuhara, Junichi Tanaka, Akane Yukimori, Shoko Ishida, Ikuko Takakura, Koki Takamatsu, Satoko Kujiraoka |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
endocrine system animal structures Organogenesis Mesenchyme Submandibular Gland Medicine (miscellaneous) Ectoderm Biology Salivary Glands General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine stomatognathic system Conditional gene knockout medicine Animals General Dentistry Salivary gland 030206 dentistry Embryonic stem cell Cell biology 030104 developmental biology medicine.anatomical_structure embryonic structures Stem cell Signal Transduction Morphogen |
| Zdroj: | Journal of Oral Biosciences. 63:8-13 |
| ISSN: | 1349-0079 |
| DOI: | 10.1016/j.job.2021.01.005 |
| Popis: | Background Organogenesis is regulated by morphogen signaling and transcription networks. These networks differ between organs, and identifying the organ-specific network is important to clarify the molecular mechanisms of development and regeneration of organs. Several studies have been conducted to identify salivary gland-specific networks using a mouse submandibular gland model. The submandibular glands (SMGs) of mice manifest as a thickening of the oral epithelium at embryonic day 11.5 and invaginate into the underlying mesenchyme. The network between Fgf10 and Sox9 is involved in SMG development in mice. Highlight Sox9, a member of the Sox family, is expressed in the SMG in mice from the embryonic stage to the adult stage, although the distribution changes during development. A null mutation of mouse Sox9 is lethal during the neonatal period due to respiratory failure, whereas deletion of Sox9 in the oral epithelium using the Cre/lox P system, can lead to smaller initial buds of SMGs in conditional knockout (cKO) mice than in normal mice. In addition, we showed that adenoviral transduction of Sox9 and Foxc1 genes into mouse embryonic stem cell–derived oral ectoderm could induce salivary gland rudiment in an organoid culture system. ChIP-sequencing revealed that Sox9 possibly regulates several tube- and branching-formation–related genes. Conclusion Sox9 may serve as an essential transcription factor for salivary gland development. The Sox9-mediated pathway can be a promising candidate for regenerating damaged salivary glands. |
| Databáze: | OpenAIRE |
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