FgHtf1 Regulates Global Gene Expression towards Aerial Mycelium and Conidiophore Formation in the Cereal Fungal Pathogen Fusarium graminearum
| Autor: | Yingzi Yun, Huawei Zheng, Dan Liu, Thomas K. Mitchell, Kai Zhang, Guangpu Li, Gaili Fan, Stephen O. Opiyo, Veena Devi Ganeshan, Guodong Lu, Zonghua Wang, Mingyang Li |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Mycelium Ecology 030306 microbiology Gene Expression Profiling RNA Conidiation RNA-Seq Spores Fungal Biology Applied Microbiology and Biotechnology Cell biology Fungal Proteins 03 medical and health sciences Fusarium Gene Expression Regulation Fungal Gene expression Environmental Microbiology Homeobox Gene Chromatin immunoprecipitation Transcription factor 030304 developmental biology Food Science Biotechnology |
| Zdroj: | Appl Environ Microbiol |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.03024-19 |
| Popis: | The homeobox gene family of transcription factors (HTF) controls many developmental pathways and physiological processes in eukaryotes. We previously showed that a conserved HTF in the plant-pathogenic fungus Fusarium graminearum, Htf1 (FgHtf1), regulates conidium morphology in that organism. This study investigated the mechanism of FgHtf1-mediated regulation and identified putative FgHtf1 target genes by a chromatin immunoprecipitation assay combined with parallel DNA sequencing (ChIP-seq) and RNA sequencing. A total of 186 potential binding peaks, including 142 genes directly regulated by FgHtf1, were identified. Subsequent motif prediction analysis identified two DNA-binding motifs, TAAT and CTTGT. Among the FgHtf1 target genes were FgHTF1 itself and several important conidiation-related genes (e.g., FgCON7), the chitin synthase pathway genes, and the aurofusarin biosynthetic pathway genes. In addition, FgHtf1 may regulate the cAMP-protein kinase A (PKA)-Msn2/4 and Ca(2+)-calcineurin-Crz1 pathways. Taken together, these results suggest that, in addition to autoregulation, FgHtf1 also controls global gene expression and promotes a shift to aerial growth and conidiation in F. graminearum by activation of FgCON7 or other conidiation-related genes. IMPORTANCE The homeobox gene family of transcription factors is known to be involved in the development and conidiation of filamentous fungi. However, the regulatory mechanisms and downstream targets of homeobox genes remain unclear. FgHtf1 is a homeobox transcription factor that is required for phialide development and conidiogenesis in the plant pathogen F. graminearum. In this study, we identified FgHtf1-controlled target genes and binding motifs. We found that, besides autoregulation, FgHtf1 also controls global gene expression and promotes conidiation in F. graminearum by activation of genes necessary for aerial growth, FgCON7, and other conidiation-related genes. |
| Databáze: | OpenAIRE |
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