Sphingomyelin synthase 2 deficiency inhibits the induction of murine colitis‐associated colon cancer
| Autor: | Makoto Taniguchi, Toshiro Okazaki, Chieko Hashizume, Takeo Kosaka, Toshio Ohnishi, Hidehiro Furumoto, Shinichi Kinami, Rongfen Gao, Jia Han |
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| Rok vydání: | 2017 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Ceramide Colorectal cancer Transferases (Other Substituted Phosphate Groups) Inflammation Lung injury Ceramides medicine.disease_cause Biochemistry Gene Expression Regulation Enzymologic Proinflammatory cytokine Mice 03 medical and health sciences chemistry.chemical_compound Genetics medicine Animals Colitis Molecular Biology Cell Proliferation Mice Knockout Chemistry Azoxymethane medicine.disease digestive system diseases Gene Expression Regulation Neoplastic 030104 developmental biology Colonic Neoplasms Cancer research medicine.symptom Carcinogenesis Signal Transduction Biotechnology |
| Zdroj: | The FASEB Journal. 31:3816-3830 |
| ISSN: | 1530-6860 0892-6638 |
| Popis: | Sphingomyelin synthase 2 (SMS2) is the synthetic enzyme of sphingomyelin (SM), which regulates membrane fluidity and microdomain structure. SMS2 plays a role in LPS-induced lung injury and inflammation; however, its role in inflammation-mediated tumorigenesis is unclear. We investigated the effect of SMS2 deficiency on dextran sodium sulfate (DSS)-induced murine colitis and found inhibition of DSS-induced inflammation in SMS2-deficient (SMS2-/-) mice. DSS treatment induced a significant increase in ceramide levels, with a decrease of SM levels in SMS2-/- colon tissue, and demonstrated attenuation of the elevation of both inflammation-related gene expression and proinflammatory cytokines and chemokines, leukocyte infiltration, and MAPK and signal transducer and activator of transcription 3 activation. After undergoing transplantation of wild-type bone marrow, SMS2-/- mice also exhibited inhibition of DSS-induced inflammation in the colon, which suggested that SMS2 deficiency in bone marrow-derived immune cells was not involved in the inhibition of colitis. Finally, in an azoxymethane/DSS-induced cancer model, SMS2 deficiency significantly decreased tumor incidence in the colon. Our results demonstrate that SMS2 deficiency inhibits DSS-induced colitis and subsequent colitis-associated colon cancer via inhibition of colon epithelial cell-mediated inflammation; therefore, inhibition of SMS2 may be a potential therapeutic target for human colitis and colorectal cancer.-Ohnishi, T., Hashizume, C., Taniguchi, M., Furumoto, H., Han, J., Gao, R., Kinami, S., Kosaka, T., Okazaki, T. Sphingomyelin synthase 2 deficiency inhibits the induction of murine colitis-associated colon cancer. |
| Databáze: | OpenAIRE |
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