Gene expression and localization of amelogenin in the rat incisor
| Autor: | Kouichi Wakao, Satoshi Sasaki, Hitoyata Shimokawa, Toshihiko Inage |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
0301 basic medicine
Cervical loop Gene Expression In situ hybridization Biology Stratum intermedium 03 medical and health sciences 0302 clinical medicine stomatognathic system Dental Enamel Proteins Amelogenesis Ameloblasts Animals RNA Messenger Rats Wistar In Situ Hybridization Stellate reticulum 030102 biochemistry & molecular biology Amelogenin Odontoblasts Inner enamel epithelium 030206 dentistry General Medicine Molecular biology Immunohistochemistry Rats Incisor Dentin Odontogenesis Cattle Ameloblast |
| Zdroj: | Advances in dental research. 10(2) |
| ISSN: | 0895-9374 |
| Popis: | Gene expression and localization of amelogenin were studied in the developing rat incisor by the methods of in situ hybridization and immunohistochemistry. ISH revealed the first expression of amelogenin mRNA in the inner enamel epithelium of the cervical loop. The signals were clearly observed in pre-ameloblasts in the region bordering on predentin formation and became more intense toward the cells on the initial enamel matrix secretion. The maximal signals were found in the cytoplasm of secretory ameloblasts. From the terminal secretion zone, the signals then became gradually weaker toward the incisal edge but were still evident in the cytoplasm of shortening, transitional ameloblasts and those at the early maturation stage. No signals were found in the cells of the stratum intermedium and stellate reticulum throughout amelogenesis. Immunohistochemistry by means of an antibody against amelogenin C-telopeptide consisting of 12 amino acids revealed immunoreaction in the secretory ameloblasts reacting to the ISH. When a polyclonal antibody against amelogenin was used, immunoreaction was found in the distal ends of ruffle-ended ameloblasts (RA) in the maturation zone. Those results indicated that amelogenin is synthesized by ameloblastic cells from the inner enamel epithelium to the early maturation stage and is then resorbed by the RA. |
| Databáze: | OpenAIRE |
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