A Modular In Vitro Platform for the Production of Terpenes and Polyketides from CO 2
| Autor: | Christoph Diehl, Srividhya Sundaram, Jan Bamberger, Tobias J. Erb, Niña Socorro Cortina, Nicole Paczia |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
food.ingredient
biocatalysis Raw material 010402 general chemistry 01 natural sciences Catalysis Terpene Polyketide chemistry.chemical_compound in vitro biochemistry food reaction cascades Biocatalysis | Hot Paper CO2 fixation 010405 organic chemistry business.industry Chemistry Communication Food additive General Medicine General Chemistry Modular design Combinatorial chemistry Communications 0104 chemical sciences Metabolic pathway Biocatalysis Organic synthesis business terpenes |
| Zdroj: | Angew Chem Int Ed Engl Angewandte Chemie (International Ed. in English) |
| ISSN: | 1521-3773 1433-7851 |
| DOI: | 10.1002/anie.202102333 |
| Popis: | A long‐term goal in realizing a sustainable biocatalysis and organic synthesis is the direct use of the greenhouse gas CO2 as feedstock for the production of bulk and fine chemicals, such as pharmaceuticals, fragrances and food additives. Here we developed a modular in vitro platform for the continuous conversion of CO2 into complex multi‐carbon compounds, such as monoterpenes (C10), sesquiterpenes (C15) and polyketides. Combining natural and synthetic metabolic pathway modules, we established a route from CO2 into the key intermediates acetyl‐ and malonyl‐CoA, which can be subsequently diversified through the action of different terpene and polyketide synthases. Our proof‐of‐principle study demonstrates the simultaneous operation of different metabolic modules comprising of up to 29 enzymes in one pot, which paves the way for developing and optimizing synthesis routes for the generation of complex CO2‐based chemicals in the future. Coupling of a synthetic CO2‐fixing cycle and a natural glyoxylate assimilation cycle with different biosynthetic pathways enables the sustainable production of terpenes and polyketides. Demonstration of this modular, multi‐enzyme in vitro platform sets the stage for a diversified synthesis of pharmaceuticals and commodity chemicals directly from CO2. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text