Mechanism of Suppression of Blood Glucose Level by Calcium Alginate in Rats
| Autor: | Takuo Ogihara, Akari Yokoyama, Hitoe Takahashi, Kentaro Yano, Mariko Koike, Yoko Idota, Takako Kato, Kazuyo Shiragami |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Blood Glucose Male medicine.medical_specialty Calcium alginate Membrane permeability Starch Alginates Pharmaceutical Science Administration Oral 03 medical and health sciences chemistry.chemical_compound Diabetes mellitus Internal medicine medicine Dietary Carbohydrates Animals Humans Glycoside Hydrolase Inhibitors Rats Wistar Alginic acid Pharmacology chemistry.chemical_classification 030109 nutrition & dietetics alpha-Glucosidases General Medicine medicine.disease Postprandial Period Rats 030104 developmental biology Postprandial Enzyme Endocrinology chemistry Caco-2 Cells Digestion |
| Zdroj: | Biologicalpharmaceutical bulletin. 41(9) |
| ISSN: | 1347-5215 |
| Popis: | Calcium alginate (Ca-Alg) is known to suppress the postprandial increase of blood glucose, and therefore may be helpful for preventing lifestyle-related diseases such as diabetes. In this work, we examined the mechanism of this effect. As α-amylase activity and α-glucosidase activity are involved in the digestion of starch, we examined the in vitro inhibitory effect of Ca-Alg on these enzymes. Ca-Alg showed little inhibition of α-amylase, but markedly inhibited α-glucosidase activity. The direct binding affinity of glucose for Ca-Alg was low. Also, Ca-Alg had essentially no effect on the membrane permeability of glucose. Therefore, we considered that the suppression of blood glucose by Ca-Alg is predominantly due to a decrease in the efficiency of starch digestion as a result of inhibition of α-glucosidase, possibly due to increased viscosity of the gastrointestinal contents. Next, we investigated the optimum amount in the diet and the optimum particle size of Ca-Alg for suppressing postprandial blood glucose level in rats orally administered a diet containing starch with various amounts and particle sizes of Ca-Alg. We found that 5% by weight of 270-mesh-pass Ca-Alg was most effective. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|