Intraoperative peritoneal washing cytology with the rapid immunoperoxidase method using microwave irradiation
| Autor: | Tohru Ichihara, Yuuki Takeuchi, Hiroshi Takagi, Toshiaki Nonami, Akimasa Nakao, Shuji Nomoto, Akio Harada |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Pathology medicine.medical_specialty CA-19-9 Antigen Immunocytochemistry Immunoenzyme Techniques Intraoperative Period Carcinoembryonic antigen Antigen Cytology Carcinoma Medicine Ascitic Fluid Humans Microwaves Aged biology Immunoperoxidase Staining and Labeling business.industry General Medicine Middle Aged medicine.disease Immunohistochemistry Peritoneal washing Staining Carcinoembryonic Antigen Pancreatic Neoplasms Biliary Tract Neoplasms Oncology biology.protein Surgery Female business |
| Zdroj: | Journal of surgical oncology. 60(1) |
| ISSN: | 0022-4790 |
| Popis: | The rapid immunocytochemical staining of gastrointestinal cancer-associated antigens, carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9), for intraoperative peritoneal washing cytology of pancreatic or biliary carcinoma can be completed in only 25 minutes by using microwave irradiation to accelerate incubation of the primary antibody. Only 3-second irradiation at 500 W for fresh smears produced specific antigen staining of malignant cells similar to that obtained with longer incubation, and the sensitivity of intraoperative cytology was greater than by the conventional staining method. The rapid immunocytochemical staining method of peritoneal washings at laparotomy can provide sensitive information for determining more aggressive therapy for intraperitoneal spread of malignant cells before closing the abdominal wall. |
| Databáze: | OpenAIRE |
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