The stability of antimycobacterial drugs in media used for drug susceptibility testing

Autor: Josien C.A. Jenniskens, Rob E. Aarnoutse, Jakko van Ingen, Esther L.M. Schoutrop, Michelle A. E. Brouwer, Johan W. Mouton, Beatriz E. Ferro
Přispěvatelé: Medical Microbiology & Infectious Diseases
Rok vydání: 2018
Předmět:
0301 basic medicine
Microbiology (medical)
medicine.drug_class
030106 microbiology
Microbial Sensitivity Tests
Pharmacology
Antimycobacterial
Mycobacterium
03 medical and health sciences
chemistry.chemical_compound
Minimum inhibitory concentration
All institutes and research themes of the Radboud University Medical Center
Drug Stability
Clarithromycin
Drug Resistance
Bacterial
polycyclic compounds
medicine
Humans
Cefoxitin
Dose-Response Relationship
Drug
business.industry
Isoniazid
General Medicine
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
Anti-Bacterial Agents
Infectious Diseases
lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]
chemistry
Amikacin
Culture Media
Conditioned
Linezolid
bacteria
Rifampin
business
Rifampicin
medicine.drug
Zdroj: Diagnostic Microbiology and Infectious Diseases, 92, 4, pp. 305-308
Diagnostic Microbiology & Infectious Disease, 92(4), 305-308. Elsevier Inc.
Diagnostic Microbiology and Infectious Diseases, 92, 305-308
ISSN: 1879-0070
0732-8893
Popis: The emergence of drug-resistant tuberculosis and disease caused by nontuberculous mycobacteria has increased the need for accurate drug susceptibility testing of mycobacteria. The stability of the tested drugs in relevant test media have been understudied. We assessed the stability of isoniazid, rifampicin, clarithromycin, linezolid and amikacin in Middlebrook 7H9 medium and that of clarithromycin, amikacin and cefoxitin in the cation-adjusted Mueller Hinton broth. We used ultra-performance liquid chromatography (UPLC) methods for rifampicin and isoniazid and a microbiological assay for rifampicin, clarithromycin, amikacin, cefoxitin and linezolid. Rifampicin and isoniazid concentrations in Middlebrook 7H9 medium had decreased by 92% and 54% after 7 days. The microbiological assay revealed decreases in drug concentration of ≥75% (rifampicin, clarithromycin, cefoxitin) and 60% (linezolid) after 14 days. With the exception of amikacin, all antimycobacterial drugs were unstable during 14 days of incubation in the preferred media for DST. Drug stability may influence minimum inhibitory concentration measurements.
Databáze: OpenAIRE
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