Stimuli-Responsive Drug Delivery of Doxorubicin Using Magnetic Nanoparticle Conjugated Poly(ethylene glycol)-g-Chitosan Copolymer
| Autor: | Hyun-Min Yoon, Min-Su Kang, Go-Eun Choi, Young-Joon Kim, Chang-Hyu Bae, Young-Bob Yu, Young-IL Jeong |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Polymers QH301-705.5 magnetic nanoparticle redox-responsiveness Mice Nude Apoptosis Article Catalysis Polyethylene Glycols Inorganic Chemistry Mice Tumor Cells Cultured Animals Humans Physical and Theoretical Chemistry Biology (General) Magnetite Nanoparticles Molecular Biology QD1-999 Spectroscopy Cell Proliferation Mice Inbred BALB C Antibiotics Antineoplastic iron-oxide nanoparticle chitosan stimuli-responsiveness Organic Chemistry General Medicine equipment and supplies Glutathione Xenograft Model Antitumor Assays Computer Science Applications Drug Liberation Chemistry Doxorubicin Colonic Neoplasms human activities |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 13169, p 13169 (2021) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 22; Issue 23; Pages: 13169 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Stimuli-responsive nanoparticles are regarded as an ideal candidate for anticancer drug targeting. We synthesized glutathione (GSH) and magnetic-sensitive nanocomposites for a dual-targeting strategy. To achieve this goal, methoxy poly (ethylene glycol) (MePEG) was grafted to water-soluble chitosan (abbreviated as ChitoPEG). Then doxorubicin (DOX) was conjugated to the backbone of chitosan via disulfide linkage. Iron oxide (IO) magnetic nanoparticles were also conjugated to the backbone of chitosan to provide magnetic sensitivity. In morphological observation, images from a transmission electron microscope (TEM) showed that IO nanoparticles were embedded in the ChitoPEG/DOX/IO nanocomposites. In a drug release study, GSH addition accelerated DOX release rate from nanocomposites, indicating that nanocomposites have redox-responsiveness. Furthermore, external magnetic stimulus concentrated nanocomposites in the magnetic field and then provided efficient internalization of nanocomposites into cancer cells in cell culture experiments. In an animal study with CT26 cell-bearing mice, nanocomposites showed superior magnetic sensitivity and then preferentially targeted tumor tissues in the field of external magnetic stimulus. Nanocomposites composed of ChitoPEG/DOX/IO nanoparticle conjugates have excellent anticancer drug targeting properties. |
| Databáze: | OpenAIRE |
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