Synthetic-Molecule/Protein Hybrid Probe with Fluorogenic Switch for Live-Cell Imaging of DNA Methylation
| Autor: | Isao Suetake, Miyako Nishiura, Maho Umeno, Norimichi Otomura, Yuichiro Hori, Ayuko Nishida, Kazuya Kikuchi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
01 natural sciences Biochemistry Catalysis 03 medical and health sciences chemistry.chemical_compound Mice Colloid and Surface Chemistry Live cell imaging Animals Epigenetics Fluorescent Dyes chemistry.chemical_classification Molecular Structure 010405 organic chemistry Biomolecule Optical Imaging Proteins General Chemistry DNA Methylation Fluorescence Small molecule 0104 chemical sciences 030104 developmental biology chemistry Molecular Probes DNA methylation Biophysics NIH 3T3 Cells DNA Macromolecule |
| Zdroj: | Journal of the American Chemical Society. 140(5) |
| ISSN: | 1520-5126 |
| Popis: | Hybrid probes consisting of synthetic molecules and proteins are powerful tools for detecting biological molecules and signals in living cells. To date, most targets of the hybrid probes have been limited to pH and small analytes. Although biomacromolecules are essential to the physiological function of cells, the hybrid-probe-based approach has been scarcely employed for live-cell detection of biomacromolecules. Here, we developed a hybrid probe with a chemical switch for live-cell imaging of methylated DNA, an important macromolecule in the repression of gene expression. Using a protein labeling technique, we created a hybrid probe containing a DNA-binding fluorogen and a methylated-DNA-binding domain. The hybrid probe enhanced fluorescence intensity upon binding to methylated DNA and successfully monitored methylated DNA during mitosis. The hybrid probe offers notable advantages absent from probes based on small molecules or fluorescent proteins and is useful for live-cell analyses of epigenetic phenomena and diseases related to DNA methylation. |
| Databáze: | OpenAIRE |
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