Sex Differences in Photic Entrainment and Sensitivity to Ethanol-Induced Chronodisruption in Adult Mice After Adolescent Intermittent Ethanol Exposure
| Autor: | Natalie M. Verbanes, Tia M. Watts, Megan O. Risinger, Jason L. Fabi, Gerneleh Paye, Christina L. Ruby, Ariana D'Angelo, Jiawen Zhang, Lauren Mabe, H. Scott Swartzwelder, Kaitlyn N. Palmer |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Aging endocrine system medicine.medical_specialty Alcohol Drinking Adult male medicine.medical_treatment Medicine (miscellaneous) Motor Activity Constant darkness Chronobiology Disorders Toxicology Article Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine mental disorders medicine Animals Circadian rhythm Saline reproductive and urinary physiology Sex Characteristics Ethanol business.industry Central Nervous System Depressants Adolescent alcohol Ethanol exposure Darkness Mice Inbred C57BL Psychiatry and Mental health 030104 developmental biology Endocrinology chemistry Photic entrainment Female business Photic Stimulation 030217 neurology & neurosurgery |
| Zdroj: | Alcoholism: Clinical and Experimental Research. 42:2144-2159 |
| ISSN: | 0145-6008 |
| DOI: | 10.1111/acer.13867 |
| Popis: | BACKGROUND: Evidence supports a role for the circadian system in alcohol use disorders, but the impact of adolescent alcohol exposure on circadian timing later in life is unknown. Acute ethanol attenuates circadian photic phase-resetting in adult, but not adolescent, rodents. However, nearly all studies have focused on males and it is unknown whether this adolescent-typical insensitivity to ethanol persists into adulthood after adolescent drinking. METHODS: Circadian activity was monitored in C57BL6/J mice receiving adolescent intermittent ethanol (AIE) exposure (15% ethanol and water every other day throughout adolescence) or water alone followed by 24 days wherein ethanol was not available (washout). Mice then received a challenge dose of ethanol (1.5 g/kg, i.p.) or saline 15 min prior to a 30-min phase-delaying light pulse, then were released into constant darkness (DD). To control for possible phase-shifting by ethanol challenge alone, a separate group of mice underwent AIE exposure (or water-only) and washout, then received an ethanol or saline injection, but did not receive a light pulse prior to DD. RESULTS: Striking sex differences in nearly all measures of circadian photic entrainment were observed during adolescence but AIE effects were subtle and few. Only ethanol-naïve adult male mice showed attenuated photic phase-shifts with ethanol challenge, while all other groups showed normal phase-resetting responses to light. AIE-exposed females showed a persistent delay in activity offset. CONCLUSIONS: Adult male AIE-exposed mice retained adolescent-like insensitivity to ethanol-induced suppression of photic phase-resetting, suggesting AIE-induced ‘lock-in’ of an adolescent behavioral phenotype. Adult AIE-exposed females showed delayed initiation of the rest phase. Our results also indicate that intermittent ethanol drinking has subtle effects on circadian activity in mice during adolescence that differ from previously reported effects on adult males. The observed sex differences in circadian activity, ethanol consumption and preference, and responses to ethanol challenge merit future mechanistic study. |
| Databáze: | OpenAIRE |
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