The effect of ω-3 polyunsaturated fatty acids on the liver lipidome, proteome and bile acid profile: parenteral versus enteral administration
| Autor: | Nikola Daskova, Kamila Bechynska, Miriam Bratova, Marie Heczkova, Nikola Vrzáčková, Jiri Suttnar, Lenka Bartonova, Jaroslav Zelenka, Helena Dankova, Alzbeta Hlavackova, Zuzana Berková, Katerina Podzimkova, Karel Harant, Jana Hajslova, Vit Kosek, Radislav Sedlacek, Monika Cahova |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Parenteral Nutrition Proteome lcsh:Medicine Pharmacology Enteral administration 0302 clinical medicine Malondialdehyde lcsh:Science Phospholipids chemistry.chemical_classification Multidisciplinary Bile acid Chemistry Lipidome Lipids Eicosapentaenoic Acid Liver Fatty Acids Unsaturated 030211 gastroenterology & hepatology lipids (amino acids peptides and proteins) Emulsions Polyunsaturated fatty acid Docosahexaenoic Acids medicine.drug_class Context (language use) Article Omegaven Bile Acids and Salts 03 medical and health sciences Enteral Nutrition Fish Oils Lipidomics Fatty Acids Omega-3 medicine Metabolomics Animals Rats Wistar Inflammation lcsh:R Rats Soybean Oil Oxygen Oxidative Stress 030104 developmental biology Parenteral nutrition lcsh:Q Reactive Oxygen Species |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Parenteral nutrition (PN) is often associated with the deterioration of liver functions (PNALD). Omega-3 polyunsaturated fatty acids (PUFA) were reported to alleviate PNALD but the underlying mechanisms have not been fully unraveled yet. Using omics´ approach, we determined serum and liver lipidome, liver proteome, and liver bile acid profile as well as markers of inflammation and oxidative stress in rats administered either ω-6 PUFA based lipid emulsion (Intralipid) or ω-6/ω-3 PUFA blend (Intralipid/Omegaven) via the enteral or parenteral route. In general, we found that enteral administration of both lipid emulsions has less impact on the liver than the parenteral route. Compared with parenterally administered Intralipid, PN administration of ω-3 PUFA was associated with 1. increased content of eicosapentaenoic (EPA)- and docosahexaenoic (DHA) acids-containing lipid species; 2. higher abundance of CYP4A isoenzymes capable of bioactive lipid synthesis and the increased content of their potential products (oxidized EPA and DHA); 3. downregulation of enzymes involved CYP450 drug metabolism what may represent an adaptive mechanism counteracting the potential negative effects (enhanced ROS production) of PUFA metabolism; 4. normalized anti-oxidative capacity and 5. physiological BAs spectrum. All these findings may contribute to the explanation of ω-3 PUFA protective effects in the context of PN. |
| Databáze: | OpenAIRE |
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