Isolation and thermal stabilization of bacteriocin nisin derived from whey for antimicrobial modifications of polymers
| Autor: | Alexandra Salakova, Pavlina Holcapkova, Vladimir Sedlarik, Zuzana Kolarova Raskova, Jan Drbohlav, Martina Hrabalikova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Ammonium sulfate Chromatography Materials science Article Subject Polymers and Plastics 030106 microbiology food and beverages Polyethylene glycol biochemical phenomena metabolism and nutrition lcsh:Chemical technology Antimicrobial 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Biochemistry chemistry Bacteriocin polycyclic compounds bacteria lcsh:TP1-1185 Fermentation Lactose Antibacterial activity Nisin |
| Zdroj: | International Journal of Polymer Science International Journal of Polymer Science, Vol 2017 (2017) |
| Popis: | This work describes novel alternative for extraction of bacteriocin nisin from a whey fermentation media and its stabilization by using polyethylene glycol as matrix with high practical applicability. This product was compared with commercially available nisin product stabilized by sodium chloride and nisin extracted and stabilized by using ammonium sulfate and polysorbate 80. The stability of samples was tested by means of long-term storage at -18, 4, 25, and 55 degrees C up to 165 days. The nisin content in the samples was determined by high-performance liquid chromatography and electrophoresis. In addition, effect of whey fortification with lactose on nisin production and antibacterial activity studied against Staphylococcus aureus was tested. Results show that stabilization by polyethylene glycol provides enhanced nisin activity at 55 degrees C after 14 days and long-term stability at 25 degrees C with keeping antibacterial activity. Ministry of Agriculture of the Czech Republic [QJ1310254]; Ministry of Education, Youth and Sports of the Czech Republic [LO1504]; Internal Grant Agency of Tomas Bata University in Zlin [IGA/CPS/2017/005] QJ1310254, MZe, Ministerstvo Zemědělství; LO1504, MEYS, Ministry of Education, Youth and Science; IGA/CPS/2017/005 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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