Wnt2 protein plays a role in the progression of pancreatic cancer promoted by pancreatic stellate cells
| Autor: | Jihui Hao, Chen Zheng, Yong Xu, Tiansuo Zhao, Hua Li, Huan Zhang, He Ren, Chongbiao Huang |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Fluorescent Antibody Technique Wnt2 Protein Adenocarcinoma Biology Metastasis Immunoenzyme Techniques Cell Movement WNT2 Pancreatic cancer Tumor Cells Cultured medicine Humans Neoplasm Invasiveness Epithelial–mesenchymal transition beta Catenin Cell Proliferation Neoplasm Staging Pancreatic Stellate Cells Wnt signaling pathway Hematology General Medicine Middle Aged Prognosis medicine.disease Pancreatic Neoplasms Survival Rate Oncology Lymphatic Metastasis Disease Progression Cancer research Hepatic stellate cell Female Neoplasm Grading Signal transduction Carcinoma Pancreatic Ductal |
| Zdroj: | Medical Oncology. 32 |
| ISSN: | 1559-131X 1357-0560 |
| Popis: | This study aimed to investigate the expression of Wnt2 protein in pancreatic cancer tissues and pancreatic stellate cells (PSCs), and determine its effect on the biological functions of pancreatic cancer cells. Immunohistochemistry was used to study the expression pattern of Wnt2 in pancreatic cancer tissues. The relationship between Wnt2 protein expression level and patient prognosis was analyzed. PSCs were isolated and cultured. The expression of Wnt2 in activated PSCs was investigated using Western blot and immunofluorescence. We also analyzed the effect of Wnt2 recombinant protein and stellate cell culture supernatant on the Wnt/β-catenin signaling pathway, as well as the effect of Wnt2 recombinant protein on the biological functions of pancreatic cancer cells. The expression of Wnt2 in interstitial cells of pancreatic cancer was correlated with the prognosis of pancreatic cancer. Wnt2 protein was expressed in activated PSCs. Both stellate cell culture supernatant and Wnt2 recombinant protein could activate the classic Wnt/β-catenin signaling pathway. Wnt2 protein enhanced the migration, invasion, and metastasis of pancreatic cancer cells. These results suggested that Wnt2 protein secreted by PSCs promoted the progression of pancreatic cancer by activating the classic Wnt/β-catenin signaling pathway. |
| Databáze: | OpenAIRE |
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