MiR-539 functions as a tumor suppressor in pancreatic cancer by targeting TWIST1
| Autor: | Wu Ji, Haibo Yu, Xiaodan Jin, Jing Cai, Hongliang Song, Zhongwu Ma, Bujian Pan, Ganglong Gao |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine Epithelial-Mesenchymal Transition Clinical Biochemistry Mice Nude Apoptosis Biology Pathology and Forensic Medicine law.invention Mice 03 medical and health sciences 0302 clinical medicine Cell Movement law Cell Line Tumor Pancreatic cancer microRNA medicine Animals Humans Inducer Epithelial–mesenchymal transition Molecular Biology Aged Cell Proliferation Transition (genetics) Twist-Related Protein 1 Nuclear Proteins Middle Aged In vitro experiment medicine.disease Xenograft Model Antitumor Assays Pancreatic Neoplasms MicroRNAs 030104 developmental biology Real-time polymerase chain reaction 030220 oncology & carcinogenesis Cancer research Suppressor Female Transcriptome |
| Zdroj: | Experimental and Molecular Pathology. 108:143-149 |
| ISSN: | 0014-4800 |
| DOI: | 10.1016/j.yexmp.2019.04.012 |
| Popis: | The dysregulation of microRNA (miRNA) expression has been highlighted in a variety of human malignant conditions with reports implicating a critical role in the process of tumor growth. The role of miR-539 in pancreatic cancer (PC) is yet to be fully elucidated, hence the aim of the current study was to investigate the effect of miR-539 expression in relation to a cohort of 52 PC specimens. The application of a real-time quantitative polymerase chain reaction (qRT-PCR) revealed a significantly down-regulated miR-539 level, which was accompanied by an increased TWIST1 expression in PC when compared with the controls. The in vitro experiment results demonstrated that the endogenic mimic of miR-539 significantly suppressed the growth of the xenograft tumors in PANC-1 cells, when compared to the delivery of the control miRNA and blank control. Meanwhile, the key epithelial-mesenchymal transition (EMT) inducer, TWIST1 was verified as a direct target gene of miR-539 through the application of a luciferase reporter assay. In conclusion, the results of the current study present evidence emphasizing the significance of the interactions between miR-539 and TWIST1 in the development of and progression of PC, highlighting its potential as a therapeutic target in the treatment of PC patients. |
| Databáze: | OpenAIRE |
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