The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules
| Autor: | Simcha Yagel, Vaclav Horejsi, Hagit Achdout, Noam Stern, Tal I. Arnon, Tsufit Gonen-Gross, Debra Goldman-Wohl, Roi Gazit, Ofer Mandelboim |
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| Rok vydání: | 2005 |
| Předmět: |
Immunology
Leukocyte Immunoglobulin-like Receptor B1 Human leukocyte antigen Biology Antigens CD HLA Antigens HLA-G Cell Line Tumor MHC class I Papain medicine Immunology and Allergy Humans Receptors Immunologic Cell Line Transformed HLA-G Antigens Beta-2 microglobulin Decidua Histocompatibility Antigens Class I Transfection Cell biology Killer Cells Natural medicine.anatomical_structure Cell culture embryonic structures biology.protein beta 2-Microglobulin |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 175(8) |
| ISSN: | 0022-1767 |
| Popis: | For a proper development of the placenta, maternal NK cells should not attack the fetal extravillous cytotrophoblast cells. This inhibition of maternal NK cells is partially mediated via the nonclassical MHC class I molecule HLA-G. Recently, we demonstrated that HLA-G forms disulfide-linked high molecular complexes on the surface of transfected cells. In the present study, we demonstrate that HLA-G must associate with β2m for its interaction with CD85J/leukocyte Ig-like receptor-1 (LIR-1). Although HLA-G free H chain complexes are expressed on the surface, they are not recognized and possibly interfere with CD85J/LIR-1 and HLA-G interaction. The formation of these complexes on the cell surface might represent a novel mechanism developed specifically by the HLA-G protein aimed to control the efficiency of the CD85J/LIR-1-mediated inhibition. We also show that endogenous HLA-G complexes are expressed on the cell surface. These findings provide novel insights into the delicate interaction between extravillous cytotrophoblast cells and NK cells in the decidua. |
| Databáze: | OpenAIRE |
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