Combining proteomics and lipid analysis to unravel Confidor stress response in Saccharomyces cerevisiae
Autor: | Gordana Čanadi Jurešić, Ana Katić, Deni Uršičić, Iva Justinić, Božena Ćurko-Cofek, Branka Blagović |
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Rok vydání: | 2019 |
Předmět: |
Proteomics
Proteome proteome Health Toxicology and Mutagenesis Saccharomyces cerevisiae Citric Acid Cycle yeast 010501 environmental sciences Management Monitoring Policy and Law Mitochondrion Toxicology 01 natural sciences 03 medical and health sciences Neonicotinoids 0302 clinical medicine Cytosol lipid Stress Physiological Glycolysis Pesticides 0105 earth and related environmental sciences chemistry.chemical_classification INTERDISCIPLINARY AREAS OF KNOWLEDGE. Biotechnology in Biomedicine (natural science biomedicine and healthcare bioethics area biology Gluconeogenesis stress response General Medicine biology.organism_classification Confidor Lipid Metabolism Nitro Compounds Yeast Mitochondria Citric acid cycle Enzyme chemistry Biochemistry 030220 oncology & carcinogenesis Microsome INTERDISCIPLINARNA PODRUČJA ZNANOSTI. Biotehnologija u biomedicini (prirodno područje biomedicina i zdravstvo biotehničko područje) |
Zdroj: | Environmental Toxicology Volume 35 Issue 3 |
ISSN: | 1522-7278 1520-4081 |
Popis: | The yeast Saccharomyces cerevisiae is a useful model for studying the influence of different stress factors on eukaryotic cells. In this work we used the pesticide imidacloprid, in the Confidor® formulation, as the stress factor and analyzed its influence on the metabolic activity, proteome and lipid content and composition of Saccharomyces cerevisiae yeast. During the cultivation of yeast, the lowest recommended application dose of Confidor® (0.025 %, v/v) was added to the growth media and its influence on the mitochondria, cytosol with microsomes and the whole yeast cells was monitored. The results show that under the stress provoked by the toxic effects of Confidor®, yeast cells density significantly decreased and the percentage of metabolically disturbed cells significantly increased comparing with untreated control. Also, there was a downregulation of majority of glycolytic, gluconeogenesis and TCA cycle enzymes (Fba1, Adh1, Hxk2, Tal1, Tdh1, Tdh3, Eno1) thus providing enough acetyl-CoA for the lipid restructuring and accumulation mechanism since we have found the changes in the cell and mitochondrial lipid content and FA composition. This data suggest that lipids could be the molecules that orchestrate the answer of the cells in the stress response to the Confidor® treatment. |
Databáze: | OpenAIRE |
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