Combining proteomics and lipid analysis to unravel Confidor stress response in Saccharomyces cerevisiae

Autor: Gordana Čanadi Jurešić, Ana Katić, Deni Uršičić, Iva Justinić, Božena Ćurko-Cofek, Branka Blagović
Rok vydání: 2019
Předmět:
Proteomics
Proteome
proteome
Health
Toxicology and Mutagenesis
Saccharomyces cerevisiae
Citric Acid Cycle
yeast
010501 environmental sciences
Management
Monitoring
Policy and Law
Mitochondrion
Toxicology
01 natural sciences
03 medical and health sciences
Neonicotinoids
0302 clinical medicine
Cytosol
lipid
Stress
Physiological
Glycolysis
Pesticides
0105 earth and related environmental sciences
chemistry.chemical_classification
INTERDISCIPLINARY AREAS OF KNOWLEDGE. Biotechnology in Biomedicine (natural science
biomedicine and healthcare
bioethics area
biology
Gluconeogenesis
stress response
General Medicine
biology.organism_classification
Confidor
Lipid Metabolism
Nitro Compounds
Yeast
Mitochondria
Citric acid cycle
Enzyme
chemistry
Biochemistry
030220 oncology & carcinogenesis
Microsome
INTERDISCIPLINARNA PODRUČJA ZNANOSTI. Biotehnologija u biomedicini (prirodno područje
biomedicina i zdravstvo
biotehničko područje)
Zdroj: Environmental Toxicology
Volume 35
Issue 3
ISSN: 1522-7278
1520-4081
Popis: The yeast Saccharomyces cerevisiae is a useful model for studying the influence of different stress factors on eukaryotic cells. In this work we used the pesticide imidacloprid, in the Confidor® formulation, as the stress factor and analyzed its influence on the metabolic activity, proteome and lipid content and composition of Saccharomyces cerevisiae yeast. During the cultivation of yeast, the lowest recommended application dose of Confidor® (0.025 %, v/v) was added to the growth media and its influence on the mitochondria, cytosol with microsomes and the whole yeast cells was monitored. The results show that under the stress provoked by the toxic effects of Confidor®, yeast cells density significantly decreased and the percentage of metabolically disturbed cells significantly increased comparing with untreated control. Also, there was a downregulation of majority of glycolytic, gluconeogenesis and TCA cycle enzymes (Fba1, Adh1, Hxk2, Tal1, Tdh1, Tdh3, Eno1) thus providing enough acetyl-CoA for the lipid restructuring and accumulation mechanism since we have found the changes in the cell and mitochondrial lipid content and FA composition. This data suggest that lipids could be the molecules that orchestrate the answer of the cells in the stress response to the Confidor® treatment.
Databáze: OpenAIRE
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