Murine CD8 T-cell functional avidity is stable in vivo but not in vitro: Independence from homologous prime/boost time interval and antigen density
| Autor: | Chensu Wang, Mathilde Allard, Grégory Verdeil, Michael Hebeisen, Darrell J. Irvine, Martin F. Bachmann, Nathalie Rufer, Daniel E. Speiser, Connie B. Gilfillan, Mona O. Mohsen |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Animals Antibody Formation Antigen Presentation Antigens/immunology Antigens/metabolism CD8-Positive T-Lymphocytes/immunology Cells Cultured Immunization Secondary Mice Mice Inbred C57BL Peptides/immunology Peptides/metabolism Protein Binding Receptors Antigen T-Cell/metabolism Vaccination Vaccines Subunit/immunology Vaccines Virus-Like Particle/immunology Avidity regulation Functional avidity Prime/boost T-cell receptor affinity T-cell vaccination Immunology Adaptive immunity Receptors Antigen T-Cell Priming (immunology) 610 Medicine & health Biology CD8-Positive T-Lymphocytes T‐cell receptor affinity 03 medical and health sciences 0302 clinical medicine Antigen In vivo Immunology and Allergy Cytotoxic T cell Avidity Vaccines Virus-Like Particle Antigens Basic Research Articles 030104 developmental biology Monoclonal Vaccines Subunit Research Article|Basic T‐cell vaccination Peptides CD8 030215 immunology |
| Zdroj: | European Journal of Immunology European journal of immunology, vol. 50, no. 4, pp. 505-514 Gilfillan, Connie B; Wang, Chensu; Mohsen, Mona O.; Rufer, Nathalie; Hebeisen, Michael; Allard, Mathilde; Verdeil, Grégory; Irvine, Darrell J; Bachmann, Martin F.; Speiser, Daniel E (2020). Murine CD8 T-cell functional avidity is stable in vivo but not in vitro: Independence from homologous prime/boost time interval and antigen density. European journal of immunology, 50(4), pp. 505-514. Wiley-VCH 10.1002/eji.201948355 |
| DOI: | 10.7892/boris.137014 |
| Popis: | It is known that for achieving high affinity antibody responses, vaccines must be optimized for antigen dose/density, and the prime/boost interval should be at least 4 weeks. Similar knowledge is lacking for generating high avidity T‐cell responses. The functional avidity (FA) of T cells, describing responsiveness to peptide, is associated with the quality of effector function and the protective capacity in vivo. Despite its importance, the FA is rarely determined in T‐cell vaccination studies. We addressed the question whether different time intervals for short‐term homologous vaccinations impact the FA of CD8 T‐cell responses. Four‐week instead of 2‐week intervals between priming and boosting with potent subunit vaccines in C57BL/6 mice did not improve FA. Equally, similar FA was observed after vaccination with virus‐like particles displaying low versus high antigen densities. Interestingly, FA was stable in vivo but not in vitro, depending on the antigen dose and the time interval since T‐cell activation, as observed in murine monoclonal T cells. Our findings suggest dynamic in vivo modulation for equal FA. We conclude that low antigen density vaccines or a minimal 4‐week prime/boost interval are not crucial for the T‐cell's FA, in contrast to antibody responses. Vaccination with changes in the prime/boost interval, or antigen density, did not influence the functional avidity (FA) of antigen‐specific CD8 T cells. However, a monoclonal population in vitro showed improved FA over time. These results suggest steady FA regulation in vivo to ensure equal and stable function and efficiency. |
| Databáze: | OpenAIRE |
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