Up-regulation of gut-enriched krüppel-like factor by interferon-γ in human colon carcinoma cells
| Autor: | Jue-Lon Shie, Zhi Y. Chen, Chi-Chuan Tseng |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
p53
Blotting Western Kruppel-Like Transcription Factors Biophysics Antineoplastic Agents Apoptosis Biology Transfection Biochemistry Substrate Specificity Interferon-gamma Kruppel-Like Factor 4 Downregulation and upregulation Structural Biology Tumor Cells Cultured Genetics Carcinoma medicine Protein biosynthesis Humans RNA Messenger Gut-enriched krüppel-like factor Molecular Biology Gene Messenger RNA Dose-Response Relationship Drug Cell growth Cell Biology medicine.disease Up-Regulation Cell biology DNA-Binding Proteins Kinetics Protein Biosynthesis Growth arrest Colonic Neoplasms Dactinomycin HT-29 cell Tumor Suppressor Protein p53 Cell Division Function (biology) Transcription Factors |
| Zdroj: | FEBS Letters. 477:67-72 |
| ISSN: | 0014-5793 |
| Popis: | Interferon-gamma (IFN-gamma) induces growth arrest and apoptosis of tumor cells but the mechanisms for these functions are unknown. Recently, gut-enriched krüppel-like factor (GKLF) was found to possess similar biological properties. Treatment of HT-29 cells with IFN-gamma inhibited cell proliferation and induced apoptosis, the effect was found to associate with GKLF expression. IFN-gamma stimulates GKLF mRNA and protein levels in a dose- and time-dependent manner and this process is independent of p53, occurs rapidly and does not require de novo protein synthesis indicating that GKLF is an immediate-early IFN-gamma-responsive gene. Moreover, overexpression of GKLF results in similar effect as IFN-gamma suggesting that GKLF may function as a downstream target of IFN-gamma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text