Large-scale identification of transcripts expressed in a symbiotic fungus (Termitomyces) during plant biomass degradation
| Autor: | Napavarn Noparatnaraporn, Yaovapa Taprab, Moriya Ohkuma, Toru Johjima, Toshiaki Kudo |
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| Rok vydání: | 2006 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Fungus Lignin Applied Microbiology and Biotechnology Termitomyces Gene Expression Regulation Fungal Botany Consensus sequence Biomass RNA Messenger Cellulose Gene Gene Library Expressed Sequence Tags Genetics Expressed sequence tag biology Reverse Transcriptase Polymerase Chain Reaction cDNA library Gene Expression Profiling Fungal genetics food and beverages RNA Fungal Sequence Analysis DNA General Medicine biology.organism_classification Enzymes Biodegradation Environmental Agaricales Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 73:195-203 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-006-0570-8 |
| Popis: | Fungus-growing termites have a symbiotic relationship with the basidiomycetes of the genus Termitomyces. This symbiotic system is able to degrade dead plant material efficiently. We conducted expressed sequence tag (EST) analysis of a symbiotic Termitomyces fungus degrading plant material in a field nest of the termite Macrotermes gilvus. A subtractive cDNA library was also investigated to facilitate the discovery of genes expressed specifically under the symbiotic conditions. A total of 2,613 ESTs were collected and resulted in 1,582 nonredundant tentative consensus sequences, of which approximately 59% showed significant similarity to known protein sequences. A number of homologous sequences to genes involved in plant cell wall degradation were identified and a majority of them encoded putative pectinolytic enzymes. Real-time quantitative reverse transcriptase polymerase chain reaction analyses confirmed significant upregulation of putative stress response genes under symbiotic conditions. The present ESTs database provides a valuable resource for molecular biological study of plant material degradation in the symbiosis between termites and fungi. |
| Databáze: | OpenAIRE |
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