Increased survival of preimplantation mouse embryos in medium with recombinant cytokine LIF

Autor:	I. V. Kapralova, L. M. Mezhevikina, E. E. Fesenko, V. V. Fedorova
Rok vydání:	2006
Předmět:	endocrine system urogenital system medicine.medical_treatment Uterus Trophoblast Embryo Biology Andrology medicine.anatomical_structure Cytokine embryonic structures Immunology medicine Inner cell mass Oviduct Blastocyst Zona pellucida reproductive and urinary physiology Developmental Biology
Zdroj:	Scopus-Elsevier
ISSN:	1608-3326 1062-3604
DOI:	10.1134/s1062360406010073
Popis:	We studied the effects of cytokine LIF on in vitro development of 2-cell mouse embryos to the late blastocyst stage. LIF at 10 ng/ml enhanced the blastocyst formation and hatching from zona pellucida. When blastocysts were cultivated in a medium with LIF for a longer time, the trophoblast adhesive properties and proliferative activity were enhanced. In the presence of this cytokine, the trophoblast cells were attached to the substrate surface and fulfill the function of a sublayer for growth of the inner cell mass colonies with a high activity of endogenous alkaline phosphatase. Expression of LIF was detected in the oviduct and uterus epithelial tissues from day 1 until day 4 of pregnancy, thus suggesting its involvement in early development. According to the data of cultivation, cytokine LIF enhanced the adhesive properties and functional activity of the trophoblast cells, which is essential for implantation of blastocysts in the uterus.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::085c20274fb93fb82ac21260bc11f66b https://doi.org/10.1134/s1062360406010073 Zobrazit plný text záznamu Full text from SpringerLink