Regulation of Thy-1 gene expression in transgenic mice
| Autor: | Hiroyuki Nishimura, Egilde Seravalli, Jack Silver, Wolfgang J. Rettig, Joan Maccari, Tetsuro Endo, Pilar Garin Chesa, Jon W. Gordon, Tetsunori Seki |
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| Rok vydání: | 1987 |
| Předmět: |
Regulation of gene expression
Genetically modified mouse Microinjections Histocytochemistry DNA Recombinant Pair-rule gene Antibodies Monoclonal Biology Embryo Mammalian Molecular biology General Biochemistry Genetics and Molecular Biology Mice genomic DNA Gene Expression Regulation Antigens Surface Gene expression Animals Humans Thy-1 Antigens Heterologous expression Allele Gene |
| Zdroj: | Cell. 50:445-452 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/0092-8674(87)90498-3 |
| Popis: | Genomic DNA fragments encompassing the human Thy-1 or mouse Thy-1.1 gene have been microinjected into pronuclei of mouse embryos homozygous for the Thy-1.2 allele. In the resulting transgenic mice, the human gene is expressed in a pattern characteristic of normal human tissues, and is not influenced by the pattern of endogenous mouse Thy-1 expression. The mouse Thy-1.1 gene fragment is expressed in a pattern typical of mouse Thy-1, although it is more limited in its distribution. The results indicate the presence of multiple cis-acting regulators of Thy-1 gene expression that have changed in both their character and arrangement over the course of Thy-1 gene evolution. |
| Databáze: | OpenAIRE |
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