Biochemical Model for Inflammation of the Brain: The Effect of Iron and Transferrin on Monocytes and Lipid Peroxidation
| Autor: | Willie M. U. Daniels, Hon D, Felix Potocnik, Jacobus Hendricks, Johann M. van Zyl, Susan J. van Rensburg, Rajiv T Erasmus |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Fever Free Radicals Cell Survival Iron Inflammation Biochemistry Mass Spectrometry Monocytes Lipid peroxidation Cellular and Molecular Neuroscience chemistry.chemical_compound Internal medicine medicine Humans Free-radical theory of aging chemistry.chemical_classification Chemistry Monocyte Transferrin Acute-phase protein Metabolism Chromatography Ion Exchange Zinc medicine.anatomical_structure Endocrinology Encephalitis Female Lipid Peroxidation Neurology (clinical) medicine.symptom Nephelometry Copper Aluminum |
| Zdroj: | Metabolic Brain Disease. 19:97-112 |
| ISSN: | 0885-7490 |
| DOI: | 10.1023/b:mebr.0000027421.33085.8b |
| Popis: | Cerebral inflammation plays a role in diseases such as multiple sclerosis (MS), Alzheimer's disease (AD), and depression. Iron is involved in infection and inflammation through free radical production. Theoretically transferrin should prohibit iron from participating in oxidative reactions, but transferrin has also been found to promote free radical damage. We reported previously that isolation of transferrin from plasma by ion exchange column chromatography produced a broad pink protein band that subsequently separated on a gel filtration column into three proteins containing many metals. In this study some properties of the three proteins were studied in 20 volunteers. Protein 3 (identified as transferrin by nephelometry) contained the most iron while Protein 1 (called "toxiferrin") contained significantly less iron (p < 0.00001). Plasma from volunteers obtained under conditions of infection/inflammation with fever (n = 5) had a significantly increased toxiferrin to transferrin ratio compared to healthy volunteers (n = 15; p < 0.001). In vitro, Protein 2 and transferrin inhibited lipid peroxidation, while toxiferrin (possibly a protease degradation product of transferrin), enhanced lipid peroxidation. Also, toxiferrin (1 mg/mL) caused a significant increase in viability of monocytes as measured by the 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyl tetrazolium bromide (MTT) reduction test, as well as the morphological transformation of monocytes to macrophages. |
| Databáze: | OpenAIRE |
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