Profiles of Pulp Infiltrating Lymphocytes at Various Times Throughout Feather Regeneration in Smyth Line Chickens with Vitiligo
| Autor: | J. R. Smyth, S. Shresta, G. F. Erf |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cytotoxicity Immunologic animal structures Receptors Antigen T-Cell alpha-beta Lymphocyte T cell Immunology Vitiligo CD8-Positive T-Lymphocytes Biology Autoimmune Diseases Mice Antigen medicine Animals Regeneration Immunology and Allergy Cytotoxic T cell Poultry Diseases B cell B-Lymphocytes T lymphocyte Feathers Molecular biology Lymphocyte Subsets medicine.anatomical_structure Feather visual_art visual_art.visual_art_medium Melanocytes Chickens CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Autoimmunity. 25:193-201 |
| ISSN: | 1607-842X 0891-6934 |
| Popis: | Smyth line (SL) chickens develop a spontaneous, autoimmune, posthatch loss of pigment cells (vitiligo) in regenerating feather tissue. Smyth line vitiligo (SLV) is associated with lymphocyte infiltrations prior to and throughout the development of the disorder. It was the purpose of this study to determine the type, relative amounts, and proportions of pulp-infiltrating lymphocytes at various times throughout the growth of regenerating feathers. Feathers were plucked from 8-week-old chickens with and without SLV. Feather pulp cell suspensions were prepared when the regenerating feathers were 2, 3, 4, and 6 weeks of age. Cells were fluorescently labeled using a panel of mouse monoclonal antibodies specific for chicken lymphocytes. Both T and B cells infiltrated the feather pulp of chickens with SLV. T cell levels remained elevated throughout the 6 weeks of feather growth, while B cell levels steadily declined to control levels over the same time. The pulp-infiltrating cells were primarily T cells with an alphabeta T cell receptor expressing the Vbeta1 gene (TCR2+). The ratio between CD4+ and CD8+ cells was 1.42 and 0.75 in 2- and 6-week-old regenerating feathers from chickens with autoimmune SLV, respectively. In non-vitiliginous chickens this ratio was always near 1. These data suggest that TCR2+ T cells play an important role in SLV. CD4+ cells may play a recruiting/activating role, whereas CD8+ cells may have cytotoxic activity specifically directed against melanocytes. Additionally, this is the first report demonstrating the infiltration of B cells into the feather pulp of vitiliginous chickens. These B cells may directly/indirectly contribute to melanocyte destruction in SLV. |
| Databáze: | OpenAIRE |
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