Constant Levels of Tau Phosphorylation in the Brain of htau Mice
| Autor: | Magdalena Daurer, Saioa Alzola Aldamizetxebarria, Birgit Hutter-Paier, Stefanie Flunkert, Tina Loeffler, Joerg Neddens |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Tau pathology mouse model Hippocampus Biology lcsh:RC321-571 genetic background 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Age groups Internal medicine medicine human tau lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Molecular Biology Pathological immunofluorescent labelling phosphorylation tauopathies Brief Research Report Cortex (botany) Young age 030104 developmental biology Endocrinology Tau phosphorylation Phosphorylation progression Alzheimer’s disease 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Frontiers in Molecular Neuroscience Frontiers in Molecular Neuroscience, Vol 13 (2020) |
| ISSN: | 1662-5099 |
| Popis: | Excessive tau phosphorylation is the hallmark of tauopathies. Today's research thus focusses on the development of drugs targeting this pathological feature. To test new drugs in preclinical studies, animal models are needed that properly mimic this pathological hallmark. The htau mouse is a well-known model expressing human but lacking murine tau, allowing to evaluate the efficacy of tau modifying compounds without interference from murine tau. Htau mice are well-characterized for tau pathology at older age, although it is often not specified on which genetic background analyzed animals were bred. Since it was shown that the genetic background can influence the pathology, we evaluated the phosphorylation status of young and adult htau mice on a C57BL/6J background by analyzing ptau Ser202 and ptau Ser396 levels in the cortex and hippocampus of 3 and 12 month old animals by immunofluorescent labelling. Additionally, we evaluated total tau, ptau Thr231 and ptau Thr181 in the soluble and insoluble brain fraction of 3-15 month old htau mice by immunosorbent assay. Our results show that ptau levels of all analyzed residues and age groups are similar without strong increases over age. These data show that tau is already phosphorylated at the age of 3 months suggesting that phosphorylation starts even earlier. The early start of tau phosphorylation in htau mice enables the use of these mice for efficacy studies already at very young age. |
| Databáze: | OpenAIRE |
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