Dietary sucrose is essential to the development of liver injury in the methionine-choline-deficient model of steatohepatitis
| Autor: | Raymond Ng, Jim Yan, Jacquelyn J. Maher, Hisanobu Ogata, Michael K. Pickens, Carine Beysen, Scott M. Turner, James P. Grenert |
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| Rok vydání: | 2009 |
| Předmět: |
Blood Glucose
Male Sucrose medicine.medical_specialty QD415-436 Biology Polymerase Chain Reaction Biochemistry Mice Liver disease chemistry.chemical_compound Methionine Endocrinology Internal medicine In Situ Nick-End Labeling medicine Animals chemistry.chemical_classification Triglyceride Body Weight Fatty Acids Fatty liver apoptosis Dietary Sucrose nutritional and metabolic diseases Fatty acid Cell Biology medicine.disease Lipids eye diseases Choline Deficiency Fatty Liver de novo lipogenesis Liver chemistry Lipogenesis fatty acid Lipid Peroxidation Steatohepatitis Steatosis Research Article |
| Zdroj: | Journal of Lipid Research, Vol 50, Iss 10, Pp 2072-2082 (2009) |
| ISSN: | 0022-2275 |
| Popis: | Methionine-choline-deficient (MCD) diets cause steatohepatitis in rodents and are used to study the pathophysiology of fatty liver disease in human beings. The most widely used commercial MCD formulas not only lack methionine and choline but also contain excess sucrose and fat. The objective of this study was to determine whether dietary sucrose in the MCD formula plays a role in the pathogenesis of MCD-related liver disease. We prepared two custom MCD formulas, one containing sucrose as the principal carbohydrate and the other substituting sucrose with starch. Mice fed the sucrose-enriched formula developed typical features of MCD-related liver disease, including hepatic steatosis, hepatocellular apoptosis, alanine aminotransferase elevation, lipid peroxidation, and hepatic inflammation. In contrast, mice fed MCD-starch were significantly protected against liver injury. MCD-sucrose and MCD-starch mice displayed identical diet-related abnormalities in hepatic fatty acid uptake and triglyceride secretion. Hepatic de novo lipogenesis and triglyceride synthesis, however, were 2 times higher in MCD-sucrose mice than MCD-starch mice (P < 0.01). Hepatic lipid analysis revealed accumulation of excess saturated fatty acids in MCD-sucrose mice that correlated with hepatocellular injury. Overall, the results indicate that dietary sucrose is critical to the pathogenesis of MCD-mediated steatohepatitis. They suggest that saturated fatty acids, which are products of de novo lipogenesis, are mediators of hepatic toxicity in this model of liver disease. |
| Databáze: | OpenAIRE |
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