Catalytic effects of elongation factor Ts on polypeptide synthesis
| Autor: | Charles G. Kurland, Måns Ehrenberg, Tarmo Ruusala |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
chemistry.chemical_classification
Elongation factor Ts Translation system General Immunology and Microbiology General Neuroscience Kinetics Articles Biology Bioinformatics General Biochemistry Genetics and Molecular Biology Catalysis chemistry Biophysics Protein biosynthesis Nucleotide EF-Ts Molecular Biology EF-Tu |
| Zdroj: | The EMBO journal. 1(1) |
| ISSN: | 0261-4189 |
| Popis: | The kinetic parameters which characterize the interaction between elongation factor Tu (EF-Tu) and elongation factor Ts (EF-Ts) have been determined in a poly(uridylic acid)-primed translation system. The EF-Ts catalyzed release of GDP from EF-Tu was measured independently in a nucleotide exchange assay. We conclude that the rate-limiting step for the EF-Tu cycle in protein synthesis in the absence of EF-Ts is the release of GDP. By adding EF-Ts the time of this step is reduced from 90 s to 30 ms. Half maximal rate is obtained at an EF-Ts concentration of 2.5 x 10−6 M. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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