Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay
| Autor: | I. Baldeh, Stanley Usen, Richard A. Adegbola, Anslem Oparaugo, Brian Greenwood, Musa Hassan-King, Charles Omosigho |
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| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
Haemophilus Infections medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Pneumococcal Infections law.invention Microbiology Haemophilus influenzae law Multiplex polymerase chain reaction Streptococcus pneumoniae Pneumonia Bacterial medicine Humans Blood culture Anaerobiosis Polymerase chain reaction biology medicine.diagnostic_test Pasteurellaceae Infant medicine.disease Streptococcaceae biology.organism_classification Virology Aerobiosis Bacterial Typing Techniques Culture Media Pneumococcal infections Child Preschool Gambia Research Article |
| Zdroj: | Journal of Clinical Microbiology. 34:2030-2032 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.34.8.2030-2032.1996 |
| Popis: | A multiplex PCR assay was developed to screen blood cultures from children in The Gambia with suspected pneumonia for the simultaneous detection of Haemophilus influenzae type b and Streptococcus pneumoniae isolates. Analysis of 295 blood cultures showed that PCR detected the organisms in all samples positive by culture in two samples infected with H. influenzae type b and four samples infected with S. pneumoniae that were culture negative, indicating that this method is sensitive for detecting these organisms in blood cultures. |
| Databáze: | OpenAIRE |
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