Retinal dysplasia in mice lacking p56lck
| Autor: | Patricia Crisanti, Bertrand Néron, J Rutin, Bernard Pessac, Marie-Chantal Marty, Blancher C, B Omri, Molina Tj |
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| Rok vydání: | 1998 |
| Předmět: |
Genetically modified mouse
Cancer Research genetic structures Gene Expression chemical and pharmacologic phenomena Biology Proto-Oncogene Mas Mice chemistry.chemical_compound Gene expression Genetics medicine Animals Photoreceptor Cells Tissue Distribution RNA Messenger Molecular Biology Retina hemic and immune systems Retinal medicine.disease Mice Mutant Strains eye diseases Cell biology medicine.anatomical_structure chemistry Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Mutation Immunology Retinal dysplasia Retinal Dysplasia sense organs Neuron Signal transduction Tyrosine kinase |
| Zdroj: | Oncogene. 16:2351-2356 |
| ISSN: | 1476-5594 0950-9232 |
| DOI: | 10.1038/sj.onc.1201761 |
| Popis: | The product of the proto-oncogene p56lck is a non-receptor tyrosine kinase member of the Src family. It is found in T cells (Marth et al., 1985, 1988) and in the mouse brain (Omri et al., 1996; Van Tan et al., 1996). In this report, we describe experiments showing that Lck is present in the mouse retina neurons. Lck gene expression was identified after isolating and sequencing the specific 5' and 3' part of the cDNA obtained by RT – PCR. In adult retina Lck immunoreactivity was most abundant in photoreceptor cells and within the outer plexiform layers. Staining was also observed in the inner nuclear and plexiform layers. In transgenic mice, the disruption of the Lck gene had serious consequences on the organization of the retina causing retinal dysplasia. These mice have partial retinal detachment with infolding and rosette formation in the photoreceptor sheet. These retinal abnormalities observed in Lck deficient mice lead to the loss of normal architecture of the photoreceptor and the inner nuclear layers, and provide an important role of Lck protein in the retina development. The lack of the Lck protein produces a spectrum of retinal pathology that resembles human retinopathy of prematurity (ROP). |
| Databáze: | OpenAIRE |
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