Cytoglobin/STAP, its unique localization in splanchnic fibroblast-like cells and function in organ fibrogenesis

Autor: Norifumi Kawada, Shigeru Saeki, Yasuyuki Shimahara, Shuichi Seki, Kazuki Nakatani, Hiroaki Okuyama, Katsutoshi Yoshizato, Yuji Nakajima, Dong-Ho Kim
Rok vydání: 2003
Předmět:
Hemeproteins
Male
endocrine system
Pathology
medicine.medical_specialty
Kupffer Cells
Becaplermin
Biology
Transfection
Collagen Type I
Pathology and Forensic Medicine
Rats
Sprague-Dawley
Transforming Growth Factor beta1
Mice
Transforming Growth Factor beta
medicine
Animals
RNA
Messenger
Rats
Wistar
Fluorescent Antibody Technique
Indirect
Vitamin A
Fibroblast
Pancreas
Molecular Biology
Platelet-Derived Growth Factor
Cytoglobin
nutritional and metabolic diseases
Proto-Oncogene Proteins c-sis
Cell Biology
Fibrosis
Cell function
Globins
Rats
Specific Pathogen-Free Organisms
Cell biology
Disease Models
Animal
medicine.anatomical_structure
Pancreatitis
Peroxidases
Chronic Disease
NIH 3T3 Cells
cardiovascular system
Kidney Diseases
Splanchnic
Biomarkers
Function (biology)
circulatory and respiratory physiology
Zdroj: Laboratory Investigation. 84:91-101
ISSN: 1530-0307
0023-6837
DOI: 10.1038/sj.labinvest.3700013
Popis: Cytoglobin/stellate cell activation-associated protein (Cygb/STAP) consists of a new class of hexacoordinate globin superfamily, which was recently discovered by a proteome analysis on the rat hepatic stellate cells. Unlike haemoglobin, myoglobin, and neuroglobin, Cygb/STAP is ubiquitously expressed in several organs, although its detailed localization has not been clarified. Immunohistochemistry and immunoelectron microscopy revealed that Cygb/STAP is uniquely localized in fibroblast-like cells in splanchnic organs, namely the vitamin A-storing cell lineage, but neither in epithelial cells, endothelial cells, muscle cells, blood cells, macrophages, nor dermal fibroblasts. The expression of Cygb/STAP was upregulated in fibrotic lesions of the pancreas and kidney in which activated fibroblast-like cells or myofibroblasts are known to increase in number. In cultured hepatic stellate cells, Cygb/STAP expression was augmented by the stimulation with sera, platelet-derived growth factor-BB, and transforming growth factor-beta 1. Overexpression of Cygb/STAP in NIH 3T3 cells induced the cells to lessen migratory activities and increase the expression of collagen alpha1(I) mRNA. These results indicate that Cygb/STAP is a tissue globin uniquely localized in splanchnic fibroblastic cell lineage and may play a role in fibrotic organ disorder.
Databáze: OpenAIRE
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