Dextran-based tube-guides for the regeneration of the rat sciatic nerve after neurotmesis injury
| Autor: | Luisa Muratori, Giulia Ronchi, Sílvia Santos Pedrosa, Arménio C. Serra, Ana Lúcia Luís, S. Geuna, José D. Santos, Ana C. Fonseca, Ana Rita Caseiro, Ana Catarina Da Silva Pinho, Mariana Vieira Branquinho, Rui Damásio Alvites, Isabel Pires, Justina Prada, Ana Colette Maurício, Jorge F. J. Coelho |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Biomedical Engineering Biocompatible Materials 02 engineering and technology Constriction Neurotmesis Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Lactones Suture (anatomy) In vivo medicine Animals Trauma Nervous System General Materials Science Caproates 030304 developmental biology 0303 health sciences Chemistry Guided Tissue Regeneration Regeneration (biology) Skeletal muscle Dextrans 021001 nanoscience & nanotechnology Sciatic Nerve Nerve Regeneration Rats Dextran medicine.anatomical_structure Sciatic nerve 0210 nano-technology Biomedical engineering |
| Popis: | In this work, dextran-based nerve tube-guides were prepared, characterized and used in a standardized animal model of neurotmesis injury. Non-porous and porous transparent tube-guides were obtained by photocrosslinking of two co-macromonomers based on dextran and poly(e-caprolactone) (PCL). Swelling capacity of the tube-guides ranged from 40–60% with no visible constriction of their inner diameter. In vitro hydrolytic degradation tests showed that the tube-guides maintained their structural integrity up to 6 months. The in vivo performance of the tube-guides was evaluated by entubulation of the rat sciatic nerve after a neurotmesis injury, with a 10 mm-gap between the nerve stumps. The results showed that the tube-guides were able to promote the regeneration of the nerve in a similar manner to what was observed with conventional techniques (nerve graft and end-to-end suture). Stereological analysis proved that nerve regeneration occurred, and both tube-guides presented fibre diameter and g-ratio closer to healthy sciatic nerves. The histomorphometric analysis of Tibialis anterior (TA) skeletal muscle showed decreased neurogenic atrophy in the porous tube-guides treated group, presenting measurements that are similar to the uninjured control. |
| Databáze: | OpenAIRE |
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