Integrating targeted glycomics and untargeted metabolomics to investigate the processing chemistry of herbal medicines, a case study on Rehmanniae Radix
| Autor: | Su-Min Duan, Xiu-Yang Li, Li Zhou, Hong Shen, Qian Mao, Ming Kong, Shan-Shan Zhou, Jun Xu, Jin-Di Xu, Song-Lin Li |
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| Rok vydání: | 2016 |
| Předmět: |
Herbal Medicine
Carbohydrates Secondary Metabolism complex mixtures 01 natural sciences Biochemistry Analytical Chemistry Glycomics Metabolome Metabolomics Desiccation Chromatography Plants Medicinal Traditional medicine 010405 organic chemistry Chemistry 010401 analytical chemistry Organic Chemistry Rehmanniae Radix General Medicine Combinatorial chemistry Glycome 0104 chemical sciences Rehmannia Steam Untargeted metabolomics Medicinal herbs Drugs Chinese Herbal |
| Zdroj: | Journal of Chromatography A. 1472:74-87 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2016.10.043 |
| Popis: | It is well-recognized that multiple components, the majority of which are secondary metabolites and carbohydrates, collectively contribute to the therapeutic effects of herbal medicines. The chemical characterization of herbal medicines has focused extensively on secondary metabolites but has largely overlooked carbohydrates. Here, we proposed an integrated chromatographic technique based targeted glycomics and untargeted metabolomics strategy simultaneously determining carbohydrates and secondary metabolites for the overall chemical profiling of herbal medicines; this strategy was successfully exemplified in an investigation of processing chemistry of Rehmanniae Radix (RR), a Chinese medicinal herb. It was demonstrated that the integrated strategy holistically illuminated the variations in the glycome and metabolome of RR samples processed by the traditionally-adopted nine cycles of steaming and drying, and further elucidated the processing-induced chemical transformation mechanisms of carbohydrates and secondary metabolites, and thereby revealed the inherent chemical connections between carbohydrates and secondary metabolites. The result suggested that the proposed strategy meets the technical demands for the overall chemical characterization of herbal medicines, and therefore could serve as a powerful tool for deciphering the scientific basis of herbal medicines. |
| Databáze: | OpenAIRE |
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