Synchronized Fast SPE and UFLC Methods for the Analyses of Eight Antidiabetic Drugs in Human Plasma
| Autor: | Arvind Kumar Jain, Imran Ali, Zeid A. ALOthman, Kamlesh K. Dutta, Abdulrahman Al-Warthan |
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| Rok vydání: | 2016 |
| Předmět: |
Population
02 engineering and technology 01 natural sciences Column chromatography Drug Discovery medicine Humans Hypoglycemic Agents Gliclazide Vildagliptin Solid phase extraction education Chromatography High Pressure Liquid education.field_of_study Chromatography Chemistry Elution 010401 analytical chemistry Organic Chemistry Solid Phase Extraction General Medicine Equipment Design 021001 nanoscience & nanotechnology Repaglinide 0104 chemical sciences Computer Science Applications Glimepiride Drug Monitoring 0210 nano-technology medicine.drug |
| Zdroj: | Combinatorial chemistryhigh throughput screening. 20(3) |
| ISSN: | 1875-5402 |
| Popis: | Aim and Objective: The population of diabetic patients is rapidly increasing globally. The treatment of these patients is a complex phenomenon due to the use of the different drugs. The present article reports a synchronized fast SPE-UFLC separation of eight antidiabetic drugs in human plasma. Inexpensive solid phase extraction (SPE) and ultra fast liquid chromatography (UFLC) methods were presented for monitoring of eight antidiabetic drugs in human plasma. The separated drugs include metformin HCl, vildagliptin, gliclazide, linagliptin, sitagliptin, pioglitazone, glimepiride and repaglinide plasma sample. Material and Method: The column used was a Sunshell C18 (150 x 4.6 mm, 2.6 μm) with eluent of acetate buffer (0.05% TEA in 0.05 M NH4Ac of pH 7.0 with H 3PO4) - ACN (60 : 40, v/v). The flow rate was 1.0 mL/min with a detection wavelength of 210 nm and column temp. of 45±1oC. These drugs were extracted from human plasma using Sep-Pac C18 cartridge. Phosphate buffer (25 mM; pH 7.0) containing these drugs were allowed to pass through cartridge at 0.1 mL/min flow rate. The adsorbed drugs on C18 cartridge were eluted by methanol at 1.0 mL/min flow rate. Results: The values for the retention, separation and resolution factors were ranged from 0.072 to 9.140, 1.443 to 4.208 and 2.147 to 18.652, correspondingly. The percent recoveries for these drugs in the standard laboratory samples prepared in water ranged from 77 to 88%. These values in plasma samples ranged from 10 to 22%. Conclusion: The validated method was fruitfully adopted to analyze these drugs in human plasma for the clinical monitoring of these drugs. |
| Databáze: | OpenAIRE |
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