Charcot-Leyden crystal protein distribution in basophils and its absence in mast cells that differentiate from human umbilical cord blood precursor cells cultured in murine fibroblast culture supernatants or in recombinant human c-kit ligand
| Autor: | H Mitsui, Ann M. Dvorak, Linda Letourneau, E A Albee, T Ishizaka, Steven J. Ackerman |
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| Rok vydání: | 1994 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Cellular differentiation Basophil Biology Ligands Peripheral blood mononuclear cell 3T3 cells Mice Proto-Oncogene Proteins Receptors Colony-Stimulating Factor medicine Animals Humans Mast Cells Microscopy Immunoelectron Cells Cultured Glycoproteins Mice Inbred BALB C Receptor Protein-Tyrosine Kinases Cell Differentiation Immunogold labelling 3T3 Cells Eosinophil Fibroblasts Mast cell Fetal Blood Hematopoietic Stem Cells Molecular biology Immunohistochemistry Recombinant Proteins Basophils Culture Media Interleukin 33 Proto-Oncogene Proteins c-kit medicine.anatomical_structure Rabbits Anatomy Lysophospholipase |
| Zdroj: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 42(2) |
| ISSN: | 0022-1554 |
| Popis: | Suspension cultures of human umbilical cord blood mononuclear cells supplemented with c-kit ligand-containing additives give rise to a mixture of cells belonging to several lineages. Among those that differentiate in quantity are mature basophils, immature mast cells, and neutrophilic myelocytes. We used an ultrastructural immunogold method to detect the Charcot-Leyden crystal (CLC) protein, an eosinophil- and basophil-specific protein, to study cells that were obtained at sequential times from 3 to 14 weeks in culture. Basophils (and eosinophils, which were present in smaller numbers) labeled for the CLC protein; mast cells did not. The labeled basophil subcellular sites included formed intragranular, cytoplasmic and nuclear CLCs, cytoplasmic particle-filled and homogeneously dense granules, cytoplasm, nucleus, plasma membrane, and cytoplasmic and Golgi area vesicles. Individual basophil ultrastructural phenotypes similar to those associated with stimulated release and recovery reactions showed the expected variations in the gold-labeled subcellular compartments. Macrophages also were labeled for CLC protein within endocytotic-lysosomal structures; neutrophilic myelocytes did not contain CLC protein. On the basis of findings reported here, the combined ultrastructural morphology and immunogold phenotyping of cells differentiating in c-kit ligand-supplemented cultures allows accurate lineage assignment of the developing cells. |
| Databáze: | OpenAIRE |
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