Metabolic rearrangements in imbibed maize (Zea mays L) embryos in the presence of oxidative stressors
| Autor: | Camila J. Moratto, Liliana B. Pena, Martin Aran, Jorge M. Vázquez-Ramos, Andrea A.E. Méndez, Susana M. Gallego, Carolina L. Matayoshi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antioxidant Physiology medicine.medical_treatment Germination Plant Science Oxidative phosphorylation medicine.disease_cause Zea mays 01 natural sciences Antioxidants 03 medical and health sciences Genetics medicine chemistry.chemical_classification Reactive oxygen species Chemistry Water Hydrogen Peroxide Metabolism Amino acid De novo synthesis Oxidative Stress 030104 developmental biology Biochemistry Seeds Imbibition Reactive Oxygen Species Oxidation-Reduction Oxidative stress 010606 plant biology & botany |
| Zdroj: | Plant Physiology and Biochemistry. 155:560-569 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2020.08.029 |
| Popis: | Cadmium (Cd) is a metal known to generate oxidative stress in plants and may be particularly harmful during germination. Herein, the growth and metabolic rearrangements of maize embryo axes subjected during the imbibition stage to Cd ions and other two well-known oxidative stressors, methyl viologen (MV) and hydrogen peroxide (H2O2), were assessed for 48 h. Similar decreases in embryo's length were detected for all stressed axes up to 48 h of imbibition. By this time, treated embryos revealed greater accumulation of reactive oxygen species (ROS) and increased levels of carbonylated and ubiquitinated proteins. The proteolytic activities were intensely enhanced in the treated axes, particularly at 48 h of imbibition, and several antioxidant enzymes were induced in most cases. NMR spectroscopy followed by principal component analysis (PCA) and hierarchical cluster analysis (HCA) showed that a large proportion of polar metabolites, mainly amino acids and organic acids, were decreased under stress conditions, while carbohydrates were increased at 48 h of imbibition, with significant increases in glucose and raffinose for treated embryos relatively to controls. We demonstrated that maize embryo axes were capable of shifting their metabolism to improve their antioxidant defense system, at the expense of their growth. Under these adverse conditions, proteolysis seems to play a key role by providing free amino acids needed for the de novo synthesis of defense-related proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect