The Expression of Presenilin 1 mRNA in Skin Fibroblasts and Brains from Sporadic Alzheimer’s Disease
| Autor: | Hiroyuki Arai, Kazuyuki Ikeda, Kenji Wada, Yoshiki Adachi, Kousaku Ohno, Yukihiro Ishikawa, Katsuya Urakami, Kenji Nakashima, Akitsugu Okada, Yuzuru Ohtsuka, Hidetada Sasaki, Hisanori Kowa, Y. Ji, Yosuke Wakutani |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Aging medicine.medical_specialty Pathology Cognitive Neuroscience Biology Presenilin Central nervous system disease Degenerative disease Alzheimer Disease Internal medicine Gene expression Presenilin-1 medicine Humans RNA Messenger Fibroblast Aged Skin Brain Chemistry Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Membrane Proteins Fibroblasts medicine.disease Reverse transcriptase Psychiatry and Mental health medicine.anatomical_structure Endocrinology Female Geriatrics and Gerontology Alzheimer's disease |
| Zdroj: | Dementia and Geriatric Cognitive Disorders. 11:245-250 |
| ISSN: | 1421-9824 1420-8008 |
| DOI: | 10.1159/000017246 |
| Popis: | We examined the expression of presenilin 1 (PS-1) mRNA in cultured skin fibroblasts taken from living patients with Alzheimer’s disease (AD) and human brains taken postmortem from AD patients by RT-PCR analysis. The donors of fibroblasts consisted of 28 cases with AD and 19 neurological patient without dementia (CTL). The brains came from 17 cases with AD and 23 cases with CTL. We found that PS-1 mRNA levels in skin fibroblasts of AD patients were significantly higher than those of CTL patients (p < 0.0001). Moreover, we found that PS-1 mRNA levels in human brains with AD were significantly higher than in those with CTL (p < 0.0001). These findings suggest that high levels of PS-1 mRNA in AD may play an important role in developing AD and that the examination of PS-1 mRNA in skin fibroblasts may be helpful for the diagnosis of AD. |
| Databáze: | OpenAIRE |
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