Microbiological and molecular assessment of bacteriophage ISP for the control of Staphylococcus aureus

Autor: Rob Lavigne, Katleen Hermans, Urszula Lipinska, Jean-Paul Pirnay, Daniel De Vos, Jean-Paul Noben, Pieter-Jan Ceyssens, Françoise Bilocq, Katrien Vandersteegen, Maia Merabishvili, Wesley Mattheus
Přispěvatelé: Fitzgerald, J Ross
Rok vydání: 2011
Předmět:
Proteomics
Bacterial Diseases
Proteome
medicine.medical_treatment
lcsh:Medicine
medicine.disease_cause
Genome
Bacteriophage
Drug Resistance
Multiple
Bacterial
Bacteriophages
Genome Sequencing
lcsh:Science
Phylogeny
Staphylococci
Multidisciplinary
Spectrometric Identification of Proteins
Genomics
Staphylococcal Infections
Bacterial Pathogens
Infectious Diseases
DNA profiling
Staphylococcus aureus
Medicine
Electrophoresis
Polyacrylamide Gel
Rabbits
Research Article
Methicillin-Resistant Staphylococcus aureus
Spectrometry
Mass
Electrospray Ionization
Phage therapy
Genotype
Infectious Disease Control
Staph Infections
Genome
Viral
Biology
Staphylococcal infections
Microbiology
Biophysical Phenomena
Host Specificity
Viral Proteins
Antibiotic resistance
medicine
Animals
Humans
lcsh:R
Virion
Biology and Life Sciences
Computational Biology
medicine.disease
biology.organism_classification
Methicillin-resistant Staphylococcus aureus
Virology
Emerging Infectious Diseases
lcsh:Q
Zdroj: PLoS ONE
PLoS ONE, Vol 6, Iss 9, p e24418 (2011)
PLOS ONE
ISSN: 1932-6203
Popis: The increasing antibiotic resistance in bacterial populations requires alternatives for classical treatment of infectious diseases and therefore drives the renewed interest in phage therapy. Methicillin resistant Staphylococcus aureus (MRSA) is a major problem in health care settings and live-stock breeding across the world. This research aims at a thorough microbiological, genomic, and proteomic characterization of S. aureus phage ISP, required for therapeutic applications. Host range screening of a large batch of S. aureus isolates and subsequent fingerprint and DNA microarray analysis of the isolates revealed a substantial activity of ISP against 86% of the isolates, including relevant MRSA strains. From a phage therapy perspective, the infection parameters and the frequency of bacterial mutations conferring ISP resistance were determined. Further, ISP was proven to be stable in relevant in vivo conditions and subcutaneous as well as nasal and oral ISP administration to rabbits appeared to cause no adverse effects. ISP encodes 215 gene products on its 138,339 bp genome, 22 of which were confirmed as structural proteins using tandem electrospray ionization-mass spectrometry (ESI-MS/MS), and shares strong sequence homology with the 'Twort-like viruses'. No toxic or virulence-associated proteins were observed. The microbiological and molecular characterization of ISP supports its application in a phage cocktail for therapeutic purposes. PC holds a postdoctoral fellowship of the 'Fonds voor Wetenschappelijk Onderzoek-Vlaanderen' (FWO-Vlaanderen, Belgium). This study was performed by members of the research community 'PhageBiotics' and was funded by the Federal Public Service of Health, Food Chain Safety and Environment, Belgium (contract RT 08/6 PHAGE). DNA fingerprinting and hybridization experiments were supported by grant MED 19 of the Royal High Institute for Defence, Belgium. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Databáze: OpenAIRE
Externí odkaz: