Unforeseen swimming and gliding mode of an insect gut symbiont, Burkholderia sp. RPE64, with wrapping of the flagella around its cell body
| Autor: | Daisuke Nakane, Yoshitomo Kikuchi, Nagisa Mikami, Takayuki Nishizaka, Yoshiaki Kinosita |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Burkholderia media_common.quotation_subject 030106 microbiology Cell Motility Insect Flagellum Microbiology Article 03 medical and health sciences Cell Movement medicine Animals Aliivibrio fischeri Ecology Evolution Behavior and Systematics Cells Cultured media_common Total internal reflection fluorescence microscope biology biology.organism_classification Bobtail squid 030104 developmental biology medicine.anatomical_structure Flagella Cell Body Biophysics Cell Migration Assays |
| Zdroj: | The ISME journal. 12(3) |
| ISSN: | 1751-7370 |
| Popis: | A bean bug symbiont, Burkholderia sp. RPE64, selectively colonizes the gut crypts by flagella-mediated motility: however, the mechanism for this colonization remains unclear. Here, to obtain clues to this mechanism, we characterized the swimming motility of the Burkholderia symbiont under an advanced optical microscope. High-speed imaging of cells enabled the detection of turn events with up to 5-ms temporal resolution, indicating that cells showed reversal motions (θ ~ 180°) with rapid changes in speed by a factor of 3.6. Remarkably, staining of the flagellar filaments with a fluorescent dye Cy3 revealed that the flagellar filaments wrap around the cell body with a motion like that of a ribbon streamer in rhythmic gymnastics. A motility assay with total internal reflection fluorescence microscopy revealed that the left-handed flagellum wound around the cell body and propelled it forward by its clockwise rotation. We also detected periodic-fluorescent signals of flagella on the glass surface, suggesting that flagella possibly contacted the solid surface directly and produced a gliding-like motion driven by flagellar rotation. Finally, the wrapping motion was also observed in a symbiotic bacterium of the bobtail squid, Aliivibrio fischeri, suggesting that this motility mode may contribute to migration on the mucus-filled narrow passage connecting to the symbiotic organ. |
| Databáze: | OpenAIRE |
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