Lycium barbarum polysaccharides inhibit proliferation and migration of bladder cancer cell lines BIU87 by suppressing Pi3K/AKT pathway
| Autor: | Mang Ke, Yong-jian Cai, Hong-Yuan Yu, Xian-Jun Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
proliferation Morpholines Down-Regulation Polysaccharide migration Pi3K/AKT 03 medical and health sciences Phosphatidylinositol 3-Kinases 0302 clinical medicine Downregulation and upregulation Cell Movement Cell Line Tumor Medicine Humans Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation chemistry.chemical_classification biology Traditional medicine Akt/PKB signaling pathway business.industry biology.organism_classification Blot Gene Expression Regulation Neoplastic 030104 developmental biology Oncology chemistry Urinary Bladder Neoplasms Chromones 030220 oncology & carcinogenesis Cancer research bladder cancer Lycium lycium barbarum polysaccharides Drug Screening Assays Antitumor Wound healing business Proto-Oncogene Proteins c-akt Research Paper Drugs Chinese Herbal Signal Transduction |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Xian-Jun Zhang 1 , Hong-Yuan Yu 1 , Yong-jian Cai 1 , Mang Ke 1 1 Department of Urology, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Linhai, Zhejiang Province, PR China Correspondence to: Mang Ke, email: kemengtaizhou@163.com Keywords: lycium barbarum polysaccharides, bladder cancer, Pi3K/AKT, proliferation, migration Received: November 15, 2016 Accepted: December 09, 2016 Published: December 15, 2016 ABSTRACT The aim of this study was to verify whether Lycium barbarum polysaccharides inhibits proliferation and migration of BIU87 cells through Pi3K/AKT pathway. Different concentrations of Lycium barbarum polysaccharides were used to incubate with BIU87cells. LY-294002 and IGF-1 were used to inhibit and activate Pi3K/AKT pathway respectively. MTT were used to investigate the proliferation of BIU87cells. Transwell chambers and wound healing were used to test the migratory ability of BIU87cells. Western blotting were used to investigate the expressions of P21,P27,MMP-2, MMP-9, AKT and p-AKT in BIU87cells. Compared with the control group, the proliferation and migration of BIU87cells and the expression of p-AKT were significantly decreased in the study group; the inhibitory effect of the downregulation of p-AKT by LY-294002on the induction of BIU87cells proliferation and migration was identical to that of Lycium barbarum polysaccharides ; upregulation of p-AKT by IGF-1 reversed the Lycium barbarum polysaccharides -induced inhibition of BIU87cells dedifferentiation. In conclusion, LBP inhibits the proliferation and migration of BIU87 cells by suppressing Pi3K/AKT signaling pathway. |
| Databáze: | OpenAIRE |
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