Sustained raloxifene release from hyaluronan-alendronate-functionalized titanium nanotube arrays capable of enhancing osseointegration in osteoporotic rabbits
Autor: | Menghuan Li, Caiyun Mu, Yan Hu, Liqi Li, Xinkun Shen, Yonglin Yu, Hao Gu, Ling Huang, Kaiyong Cai, Zhining Xia |
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Rok vydání: | 2018 |
Předmět: |
Nanotube
animal structures Materials science Cell Survival Surface Properties chemistry.chemical_element Bioengineering Nanotechnology 02 engineering and technology Microscopy Atomic Force 010402 general chemistry 01 natural sciences Bone and Bones Osseointegration Bone remodeling Biomaterials Chitosan Mice chemistry.chemical_compound Animals Hyaluronic Acid Cells Cultured Titanium Drug Carriers Nanotubes Osteoblasts Alendronate Tartrate-Resistant Acid Phosphatase technology industry and agriculture Substrate (chemistry) Cell Differentiation Prostheses and Implants X-Ray Microtomography Alkaline Phosphatase musculoskeletal system 021001 nanoscience & nanotechnology 0104 chemical sciences RAW 264.7 Cells chemistry Mechanics of Materials Raloxifene Hydrochloride Alkaline phosphatase Rabbits Implant 0210 nano-technology Nuclear chemistry |
Zdroj: | Materials Science and Engineering: C. 82:345-353 |
ISSN: | 0928-4931 |
Popis: | To enhance the localized bone remodeling at titanium-based implants under osteoporotic conditions, TiO 2 nanotube arrays (TNT) were used as nanoreserviors for raloxifene (Ral) and then covered with the hybrid multilayered coating of chitosan and alendronate grafted hyaluronic acid (HA-Aln) via a spin-assisted layer-by-layer technique. The fabrication of this system (TNT/Ral/LBL-Aln) was characterized by field emission scanning electron microscopy (SEM), atomic force microscope (AFM) and X-ray photoelectron spectroscopy (XPS), respectively. The release test showed that the composited multilayers onto Ral-loaded TiO 2 nanotube substrate (TNT/Ral) could prevent the burst release of Ral from TiO 2 nanotube arrays and maintain stable Ral concentration at the implant site even after 192 h. The TNT/Ral/LBL-Aln system demonstrated higher alkaline phosphatase (ALP) activity, mineralization capability in osteoblasts as well as lower tartrate-resistant acid phosphatase (TRAP) activity in osteoclasts compared to both bare TiO 2 nanotube and TNT/Ral substrate, respectively. Moreover, the in vivo tests of micro-CT, histological staining and push-out testing showed that TNT/Ral/LBL-Aln implant could efficiently enhance the formation of new bone around the implant and promote bone binding in osteoporotic rabbits. The study indicated the potential application of TNT/Ral/LBL-Aln system for bone remodeling under osteoporotic condition. |
Databáze: | OpenAIRE |
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