Sustained raloxifene release from hyaluronan-alendronate-functionalized titanium nanotube arrays capable of enhancing osseointegration in osteoporotic rabbits

Autor: Menghuan Li, Caiyun Mu, Yan Hu, Liqi Li, Xinkun Shen, Yonglin Yu, Hao Gu, Ling Huang, Kaiyong Cai, Zhining Xia
Rok vydání: 2018
Předmět:
Nanotube
animal structures
Materials science
Cell Survival
Surface Properties
chemistry.chemical_element
Bioengineering
Nanotechnology
02 engineering and technology
Microscopy
Atomic Force
010402 general chemistry
01 natural sciences
Bone and Bones
Osseointegration
Bone remodeling
Biomaterials
Chitosan
Mice
chemistry.chemical_compound
Animals
Hyaluronic Acid
Cells
Cultured
Titanium
Drug Carriers
Nanotubes
Osteoblasts
Alendronate
Tartrate-Resistant Acid Phosphatase
technology
industry
and agriculture
Substrate (chemistry)
Cell Differentiation
Prostheses and Implants
X-Ray Microtomography
Alkaline Phosphatase
musculoskeletal system
021001 nanoscience & nanotechnology
0104 chemical sciences
RAW 264.7 Cells
chemistry
Mechanics of Materials
Raloxifene Hydrochloride
Alkaline phosphatase
Rabbits
Implant
0210 nano-technology
Nuclear chemistry
Zdroj: Materials Science and Engineering: C. 82:345-353
ISSN: 0928-4931
Popis: To enhance the localized bone remodeling at titanium-based implants under osteoporotic conditions, TiO 2 nanotube arrays (TNT) were used as nanoreserviors for raloxifene (Ral) and then covered with the hybrid multilayered coating of chitosan and alendronate grafted hyaluronic acid (HA-Aln) via a spin-assisted layer-by-layer technique. The fabrication of this system (TNT/Ral/LBL-Aln) was characterized by field emission scanning electron microscopy (SEM), atomic force microscope (AFM) and X-ray photoelectron spectroscopy (XPS), respectively. The release test showed that the composited multilayers onto Ral-loaded TiO 2 nanotube substrate (TNT/Ral) could prevent the burst release of Ral from TiO 2 nanotube arrays and maintain stable Ral concentration at the implant site even after 192 h. The TNT/Ral/LBL-Aln system demonstrated higher alkaline phosphatase (ALP) activity, mineralization capability in osteoblasts as well as lower tartrate-resistant acid phosphatase (TRAP) activity in osteoclasts compared to both bare TiO 2 nanotube and TNT/Ral substrate, respectively. Moreover, the in vivo tests of micro-CT, histological staining and push-out testing showed that TNT/Ral/LBL-Aln implant could efficiently enhance the formation of new bone around the implant and promote bone binding in osteoporotic rabbits. The study indicated the potential application of TNT/Ral/LBL-Aln system for bone remodeling under osteoporotic condition.
Databáze: OpenAIRE
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