Production of monoclonal antibodies
| Autor: | Wayne M. Yokoyama, Michelle Christensen, Gary Dos Santos, Diane Miller, Jason Ho, Tao Wu, Michael Dziegelewski, Francisca A. Neethling |
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| Rok vydání: | 2014 |
| Předmět: |
0301 basic medicine
medicine.drug_class Immunology Cell Culture Techniques chemical and pharmacologic phenomena Methylcellulose Monoclonal antibody Cell Fusion 03 medical and health sciences Epitopes 0302 clinical medicine medicine Animals B cell Cloning B-Lymphocytes Cell fusion Hybridomas biology Chemistry Antibodies Monoclonal Molecular biology Clone Cells 030104 developmental biology medicine.anatomical_structure Immunization Cell culture Monoclonal biology.protein Antibody 030215 immunology |
| Zdroj: | Current protocols in immunologyLiterature CitedKey ReferencesInternet Resources. 102 |
| ISSN: | 1934-368X |
| Popis: | This unit describes the production of monoclonal antibodies beginning with immunization, cell fusion, and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY from StemCell Technologies). |
| Databáze: | OpenAIRE |
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